ArticlePlant physiology2009
Role of the rice hexokinases OsHXK5 and OsHXK6 as glucose sensors.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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87 citing papers in PubMed, 175 citations in OpenAlex.
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- Comparative transcriptomics of atrazine-tolerant and sensitive alfalfa varieties under atrazine stress.BMC plant biology · 2025Article
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- Genome-Wide Identification and Expression Analysis of Hexokinase Gene Family Under Abiotic Stress in Tomato.Plants (Basel, Switzerland) · 2025Article
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- Bioinformatic insights into sugar signaling pathways in sugarcane growth.Scientific reports · 2024Article
- A neutral invertase controls cell division besides hydrolysis of sucrose for nutrition during germination and seed setting in rice.iScience · 2024Article
- OsWRKY26 negatively regulates bacterial blight resistance by suppressingFrontiers in plant science · 2024Article
- Structure and Expression Analysis ofInternational journal of molecular sciences · 2023Article
- HEXOKINASE1 and glucose-6-phosphate fuel plant growth and development.Development (Cambridge, England) · 2023Article
- Genome-Wide Characterization of Soybean Hexokinase Genes Reveals a Positive Role ofPlants (Basel, Switzerland) · 2023Article
- Spatial expression patterns of genes encoding sugar sensors in leaves of C4 and C3 grasses.Annals of botany · 2023Article
- Abscisic acid and regulation of the sugar transporter gene MdSWEET9b promote apple sugar accumulation.Plant physiology · 2023Article
- Unraveling the diversity and functions of sugar transporters for sustainable management of wheat rust.Functional & integrative genomics · 2023Review
- Changing course: Glucose starvation drives nuclear accumulation of Hexokinase 2 in S. cerevisiae.PLoS genetics · 2023Article
- The Impact of Carbohydrate Management on Coleoptile Elongation in Anaerobically Germinating Seeds of Rice (Plants (Basel, Switzerland) · 2023Article
- Sucrose preferentially promotes expression ofFrontiers in plant science · 2023Article
27 more citing papers are in PubMed but not listed here.
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15 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Arabidopsis (Arabidopsis thaliana) hexokinase 1 (AtHXK1) is recognized as an important glucose (Glc) sensor. However, the function of hexokinases as Glc sensors has not been clearly demonstrated in other plant species, including rice (Oryza sativa). To investigate the functions of rice hexokinase isoforms, we characterized OsHXK5 and OsHXK6, which are evolutionarily related to AtHXK1. Transient expression analyses using GFP fusion constructs revealed that OsHXK5 and OsHXK6 are associated with mitochondria. Interestingly, the OsHXK5DeltamTP-GFP and OsHXK6DeltamTP-GFP fusion proteins, which lack N-terminal mitochondrial targeting peptides, were present mainly in the nucleus with a small amount of the proteins seen in the cytosol. In addition, the OsHXK5NLS-GFP and OsHXK6NLS-GFP fusion proteins harboring nuclear localization signals were targeted predominantly in the nucleus, suggesting that these OsHXKs retain a dual-targeting ability to mitochondria and nuclei. In transient expression assays using promoterluciferase fusion constructs, these two OsHXKs and their catalytically inactive alleles dramatically enhanced the Glc-dependent repression of the maize (Zea mays) Rubisco small subunit (RbcS) and rice alpha-amylase genes in mesophyll protoplasts of maize and rice. Notably, the expression of OsHXK5, OsHXK6, or their mutant alleles complemented the Arabidopsis glucose insensitive2-1 mutant, thereby resulting in wild-type characteristics in seedling development, Glc-dependent gene expression, and plant growth. Furthermore, transgenic rice plants overexpressing OsHXK5 or OsHXK6 exhibited hypersensitive plant growth retardation and enhanced repression of the photosynthetic gene RbcS in response to Glc treatment. These results provide evidence that rice OsHXK5 and OsHXK6 can function as Glc sensors.
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