ArticleNucleic acids research2009
Analysis of re-replication from deregulated origin licensing by DNA fiber spreading.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Oncogenic p53 induces mitotic errors in lung cancer cells by recopying DNA replication forks conferring targetable proliferation advantage.Cell death and differentiation · 2026Article
- Physiological re-replication during human stem cell differentiation.Cell death discovery · 2026Article
- RAD51 restricts DNA over-replication from re-activated origins.The EMBO journal · 2024Article
- CDT1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis.Molecular cell · 2023Article
- Polyploid Giant Cancer Cells, a Hallmark of Oncoviruses and a New Therapeutic Challenge.Frontiers in oncology · 2020Review
- Uncoupling fork speed and origin activity to identify the primary cause of replicative stress phenotypes.The Journal of biological chemistry · 2018Article
- ATR kinase regulates its attenuation via PPM1D phosphatase recruitment to chromatin during recovery from DNA replication stress signalling.Journal of biosciences · 2018Article
- Mutant p53 establishes targetable tumor dependency by promoting unscheduled replication.The Journal of clinical investigation · 2017Article
- Phosphorylation of Ku70 subunit by cell cycle kinases modulates the replication related function of Ku heterodimer.Nucleic acids research · 2016Article
- Affected chromosome homeostasis and genomic instability of clonal yeast cultures.Current genetics · 2016Article
- Early S-phase cell hypersensitivity to heat stress.Cell cycle (Georgetown, Tex.) · 2016Review
- Genome Duplication: The Heartbeat of Developing Organisms.Current topics in developmental biology · 2016Review
- Re-replication of a centromere induces chromosomal instability and aneuploidy.PLoS genetics · 2015Article
- Environmental stress induces trinucleotide repeat mutagenesis in human cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- CDK1-dependent inhibition of the E3 ubiquitin ligase CRL4CDT2 ensures robust transition from S Phase to Mitosis.The Journal of biological chemistry · 2015Article
- Deregulated origin licensing leads to chromosomal breaks by rereplication of a gapped DNA template.Genes & development · 2013Article
- HDAC3 is essential for DNA replication in hematopoietic progenitor cells.The Journal of clinical investigation · 2013Article
- Activation of a human chromosomal replication origin by protein tethering.Nucleic acids research · 2013Article
- Inhibition of histone deacetylase 3 causes replication stress in cutaneous T cell lymphoma.PloS one · 2013Article
- Flipping the switch from g1 to s phase with e3 ubiquitin ligases.Genes & cancer · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A major challenge each human cell-division cycle is to ensure that DNA replication origins do not initiate more than once, a phenomenon known as re-replication. Acute deregulation of replication control ultimately causes extensive DNA damage, cell-cycle checkpoint activation and cell death whereas moderate deregulation promotes genome instability and tumorigenesis. In the absence of detectable increases in cellular DNA content however, it has been difficult to directly demonstrate re-replication or to determine if the ability to re-replicate is restricted to a particular cell-cycle phase. Using an adaptation of DNA fiber spreading we report the direct detection of re-replication on single DNA molecules from human chromosomes. Using this method we demonstrate substantial re-replication within 1 h of S phase entry in cells overproducing the replication factor, Cdt1. Moreover, a comparison of the HeLa cancer cell line to untransformed fibroblasts suggests that HeLa cells produce replication signals consistent with low-level re-replication in otherwise unperturbed cell cycles. Re-replication after depletion of the Cdt1 inhibitor, geminin, in an untransformed fibroblast cell line is undetectable by standard assays but readily quantifiable by DNA fiber spreading analysis. Direct evaluation of re-replicated DNA molecules will promote increased understanding of events that promote or perturb genome stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.