ArticleClinical cancer research : an official journal of the American Association for Cancer Research2008
Molecular imaging of therapeutic response to epidermal growth factor receptor blockade in colorectal cancer.
Article in Clinical cancer research : an official journal of the American Association for Cancer Research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers, 1 of them a synthesis that pooled it.
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Who cites it
61 citing papers in PubMed, 1 synthesis or guideline pooled it, 104 citations in OpenAlex.
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- In vivo magnetic resonance imaging of treatment-induced apoptosis.Scientific reports · 2019Article
- Cholangiocarcinoma: molecular imaging-guided radiofrequency hyperthermia-enhanced intratumoral herpes simplex virus thymidine kinase gene therapy.American journal of cancer research · 2018Article
- Dynamic Biomarkers of Response to Antiangiogenic Therapies in Colorectal Cancer: A Review.Current pharmacogenomics and personalized medicine · 2017Review
- Multi-modality imaging-monitored creation of rat orthotopic pancreatic head cancer with obstructive jaundice.Oncotarget · 2017Article
- [Comparison of early response to combined chemotherapy gemcitabine-cisplatin in non-small cell lung cancer animal model between usingSheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2017Article
- Detection and characterization of murine colitis and carcinogenesis by molecularly targeted contrast-enhanced ultrasound.World journal of gastroenterology · 2017Article
- Optimized Translocator Protein Ligand for Optical Molecular Imaging and Screening.Bioconjugate chemistry · 2017Article
- Optical imaging probes in oncology.Oncotarget · 2016Review
- Markers of Response to Antiangiogenic Therapies in Colorectal Cancer: Where Are We Now and What Should Be Next?Clinical Medicine Insights. Oncology · 2016Review
- EGFR is not a major driver for osteosarcoma cell growth in vitro but contributes to starvation and chemotherapy resistance.Journal of experimental & clinical cancer research : CR · 2015Article
- [(18)F]-FLT PET to predict early response to neoadjuvant therapy in KRAS wild-type rectal cancer: a pilot study.Annals of nuclear medicine · 2015Article
- High-yielding, automated production of 3'-deoxy-3'-[(18)F]fluorothymidine using a modified Bioscan Coincidence FDG reaction module.Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine · 2015Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
19 authors at 3 institutions in 1 country.
Funding
Abstract
purposeTo evaluate noninvasive molecular imaging methods as correlative biomarkers of therapeutic efficacy of cetuximab in human colorectal cancer cell line xenografts grown in athymic nude mice. The correlation between molecular imaging and immunohistochemical analysis to quantify epidermal growth factor (EGF) binding, apoptosis, and proliferation was evaluated in treated and untreated tumor-bearing cohorts. EXPERIMENTAL
designOptical imaging probes targeting EGF receptor (EGFR) expression (NIR800-EGF) and apoptosis (NIR700-Annexin V) were synthesized and evaluated in vitro and in vivo. Proliferation was assessed by 3'-[18F]fluoro-3'-deoxythymidine ([18F]FLT) positron emission tomography. Assessment of inhibition of EGFR signaling by cetuximab was accomplished by concomitant imaging of NIR800-EGF, NIR700-Annexin V, and [18F]FLT in cetuximab-sensitive (DiFi) and insensitive (HCT-116) human colorectal cancer cell line xenografts. Imaging results were validated by measurement of tumor size and immunohistochemical analysis of total and phosphorylated EGFR, caspase-3, and Ki-67 immediately following in vivo imaging.
resultsNIR800-EGF accumulation in tumors reflected relative EGFR expression and EGFR occupancy by cetuximab. NIR700-Annexin V accumulation correlated with cetuximab-induced apoptosis as assessed by immunohistochemical staining of caspase-3. No significant difference in tumor proliferation was noted between treated and untreated animals by [18F]FLT positron emission tomography or Ki-67 immunohistochemistry.
conclusionsMolecular imaging can accurately assess EGF binding, proliferation, and apoptosis in human colorectal cancer xenografts. These imaging approaches may prove useful for serial, noninvasive monitoring of the biological effects of EGFR inhibition in preclinical studies. It is anticipated that these assays can be adapted for clinical use.
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