ArticleUltrasound in medicine & biology2009
Spatiotemporal effects of sonoporation measured by real-time calcium imaging.
Article in Ultrasound in medicine & biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The trial behind it
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Who cites it
27 citing papers in PubMed, 95 citations in OpenAlex.
- CaUltrasonics sonochemistry · 2025Article
- Article
- Dynamic reorganization of multivesicular bodies and exosome production impacted by sonoporation.Scientific reports · 2024Article
- Development of an In Vitro Model to Study Mechanisms of Ultrasound-Targeted Microbubble Cavitation-Mediated Blood-Brain Barrier Opening.Ultrasound in medicine & biology · 2024Article
- Article
- Landscape of Cellular Bioeffects Triggered by Ultrasound-Induced Sonoporation.International journal of molecular sciences · 2022Review
- Transient nuclear lamin A/C accretion aids in recovery from vapor nanobubble-induced permeabilisation of the plasma membrane.Cellular and molecular life sciences : CMLS · 2022Article
- Targeting Orphan G Protein-Coupled Receptor 17 with T0 Ligand Impairs Glioblastoma Growth.Cancers · 2021Article
- Lytic Release of Cellular ATP: Physiological Relevance and Therapeutic Applications.Life (Basel, Switzerland) · 2021Review
- Visualization and quantification of dynamic intercellular coupling in human embryonic stem cells using single cell sonoporation.Scientific reports · 2020Article
- Ultrasound-induced molecular delivery to erythrocytes using a microfluidic system.Biomicrofluidics · 2020Article
- Synthesis and preclinical validation of novel P2Y1 receptor ligands as a potent anti-prostate cancer agent.Scientific reports · 2019Article
- Layered acoustofluidic resonators for the simultaneous optical and acoustic characterisation of cavitation dynamics, microstreaming, and biological effects.Biomicrofluidics · 2018Article
- Low-Intensity Ultrasound Modulates CaAnnals of biomedical engineering · 2018Article
- Ultrasound Stimulation of Insulin Release from Pancreatic Beta Cells as a Potential Novel Treatment for Type 2 Diabetes.Ultrasound in medicine & biology · 2017Article
- In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications.Biomicrofluidics · 2016Review
- Mechanisms of microbubble-facilitated sonoporation for drug and gene delivery.Therapeutic delivery · 2014Review
- The induction of the apoptosis of cancer cell by sonodynamic therapy: a review.Chinese journal of cancer research = Chung-kuo yen cheng yen chiu · 2012Article
- Spatiotemporally controlled single cell sonoporation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Different effects of sonoporation on cell morphology and viability.Bosnian journal of basic medical sciences · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
To investigate the effects of sonoporation, spatiotemporal evolution of ultrasound-induced changes in intracellular calcium ion concentration ([Ca(2+)](i)) was determined using real-time fura-2AM fluorescence imaging. Monolayers of Chinese hamster ovary (CHO) cells were exposed to a 1-MHz ultrasound tone burst (0.2 s, 0.45 MPa) in the presence of Optison microbubbles. At extracellular [Ca(2+)](o) of 0.9 mM, ultrasound application generated both nonoscillating and oscillating (periods 12 to 30 s) transients (changes of [Ca(2+)](i) in time) with durations of 100-180 s. Immediate [Ca(2+)](i) transients after ultrasound application were induced by ultrasound-mediated microbubble-cell interactions. In some cases, the immediately affected cells did not return to pre-ultrasound equilibrium [Ca(2+)](i) levels, thereby indicating irreversible membrane damage. Spatial evolution of [Ca(2+)](i) in different cells formed a calcium wave that was observed to propagate outward from the immediately affected cells at 7-20 microm/s over a distance >200 microm, causing delayed transients in cells to occur sometimes 60 s or more after ultrasound application. In calcium-free solution, ultrasound-affected cells did not recover, consistent with the requirement of extracellular Ca(2+) for cell membrane recovery subsequent to sonoporation. In summary, ultrasound application in the presence of Optison microbubbles can generate transient [Ca(2+)](i) changes and oscillations at a focal site and in surrounding cells via calcium waves that last longer than the ultrasound duration and spread beyond the focal site. These results demonstrate the complexity of downstream effects of sonoporation beyond the initial pore formation and subsequent diffusion-related transport through the cellular membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.