ArticleThe EMBO journal2008
Silencing and transcriptional properties of the imprinted Airn ncRNA are independent of the endogenous promoter.
Article in The EMBO journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Maternal transmission of an Igf2r domain 11: IGF2 binding mutant allele (Igf2rScientific reports · 2019Article
- The Airn lncRNA does not require any DNA elements within its locus to silence distant imprinted genes.PLoS genetics · 2019Article
- Single cell analysis reveals the involvement of the long non-coding RNA Pvt1 in the modulation of muscle atrophy and mitochondrial network.Nucleic acids research · 2019Article
- Aberrant DNA Methylation Involved in Obese Women with Systemic Insulin Resistance.Open life sciences · 2018Article
- Antisense Activity across theNon-coding RNA · 2015Article
- Evolution of the unspliced transcriptome.BMC evolutionary biology · 2015Article
- Article
- Maternal transmission of a humanised Igf2r allele results in an Igf2 dependent hypomorphic and non-viable growth phenotype.PloS one · 2013Article
- Hidden treasures in unspliced EST data.Theory in biosciences = Theorie in den Biowissenschaften · 2012Article
- R-loop formation is a distinctive characteristic of unmethylated human CpG island promoters.Molecular cell · 2012Article
- Epigenetic mechanisms of genomic imprinting: common themes in the regulation of imprinted regions in mammals, plants, and insects.Genetics research international · 2012Article
- A downstream CpG island controls transcript initiation and elongation and the methylation state of the imprinted Airn macro ncRNA promoter.PLoS genetics · 2012Article
- Uncoupling antisense-mediated silencing and DNA methylation in the imprinted Gnas cluster.PLoS genetics · 2011Article
- The function of non-coding RNAs in genomic imprinting.Development (Cambridge, England) · 2009Review
- An in vitro ES cell imprinting model shows that imprinted expression of the Igf2r gene arises from an allele-specific expression bias.Development (Cambridge, England) · 2009Article
- Chromatin mechanisms in genomic imprinting.Mammalian genome : official journal of the International Mammalian Genome SocietyReview
- Emerging similarities in epigenetic gene silencing by long noncoding RNAs.Mammalian genome : official journal of the International Mammalian Genome SocietyReview
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Airn macro ncRNA is the master regulator of imprinted expression in the Igf2r imprinted gene cluster where it silences three flanking genes in cis. Airn transcription shows unusual features normally viewed as promoter specific, such as impaired post-transcriptional processing and a macro size. The Airn transcript is 108 kb long, predominantly unspliced and nuclear localized, with only a minority being variably spliced and exported. Here, we show by deletion of the Airn ncRNA promoter and replacement with a constitutive strong or weak promoter that splicing suppression and termination, as well as silencing activity, are maintained by strong Airn expression from an exogenous promoter. This indicates that all functional regions are located within the Airn transcript. DNA methylation of the maternal imprint control element (ICE) restricts Airn expression to the paternal allele and we also show that a strong active promoter is required to maintain the unmethylated state of the paternal ICE. Thus, Airn expression not only induces silencing of flanking mRNA genes but also protects the paternal copy of the ICE from de novo methylation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.