ArticleBlood2009
An insulator with barrier-element activity promotes alpha-spectrin gene expression in erythroid cells.
Article in Blood, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Neuronal Expression of Opioid Gene is Controlled by Dual Epigenetic and Transcriptional Mechanism in Human Brain.Cerebral cortex (New York, N.Y. : 1991) · 2018Article
- Article
- Genetic Loci implicated in erythroid differentiation and cell cycle regulation are associated with red blood cell traits.Mayo Clinic proceedings · 2012Article
- Chromatin boundaries require functional collaboration between the hSET1 and NURF complexes.Blood · 2011Article
- Role of helix-loop-helix proteins during differentiation of erythroid cells.Molecular and cellular biology · 2011Review
- Learning about genomics and disease from the anucleate human red blood cell.The Journal of clinical investigation · 2010Article
- Mutation of a barrier insulator in the human ankyrin-1 gene is associated with hereditary spherocytosis.The Journal of clinical investigation · 2010Article
- USF and NF-E2 cooperate to regulate the recruitment and activity of RNA polymerase II in the beta-globin gene locus.The Journal of biological chemistry · 2010Article
- Article
- Posttranslational modifications, localization, and protein interactions of optineurin, the product of a glaucoma gene.PloS one · 2010Article
- Defective erythropoiesis in transgenic mice expressing dominant-negative upstream stimulatory factor.Molecular and cellular biology · 2009Article
- Chromatin architecture and transcription factor binding regulate expression of erythrocyte membrane protein genes.Molecular and cellular biology · 2009Article
- In vivo expression of MHC class I genes depends on the presence of a downstream barrier element.PloS one · 2009Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Understanding mechanisms controlling expression of the alpha-spectrin gene is important for understanding erythropoiesis, membrane biogenesis, and spectrin-linked hemolytic anemia. We showed previously that a minimal alpha-spectrin promoter directed low levels of expression only in early erythroid development, indicating elements outside the promoter are required for expression in adult erythrocytes. Addition of noncoding exon 1' and intron 1' conferred a 10-fold increase in activity in reporter gene assays. In this report, we used a transgenic mouse model to show that addition of exon 1' and intron 1' to the alpha-spectrin promoter conferred tissue-specific expression of a linked (A)gamma-globin gene in erythroid cells at all developmental stages. Expression was nearly position-independent, as 21 of 23 lines expressed the transgene, and gamma-globin protein was present in 100% of erythrocytes, indicating uniform expression. Additional in vivo studies revealed that exon 1' functions as an insulator with barrier-element activity. Chromatin immunoprecipitation assays demonstrated that this region was occupied by the upstream stimulatory factors 1/2 (USF1/USF2), similar to the well-characterized chicken HS4 insulator. These data identify the first barrier element described in an erythrocyte membrane protein gene and indicate that exon 1' and intron 1' are excellent candidate regions for mutations in patients with spectrin-linked hemolytic anemia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.