ArticleThe Journal of general virology2008
The influenza A virus spliced messenger RNA M mRNA3 is not required for viral replication in tissue culture.
Article in The Journal of general virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Virus usurps alternative splicing to clear the decks for infection.Virology journal · 2023Review
- Targeting influenza A virus by splicing inhibitor herboxidiene reveals the importance of subtype-specific signatures around splice sites.Journal of biomedical science · 2023Article
- A Glu-Glu-Tyr Sequence in the Cytoplasmic Tail of the M2 Protein Renders Influenza A Virus Susceptible to Restriction of the Hemagglutinin-M2 Association in Primary Human Macrophages.Journal of virology · 2022Article
- Accessory Gene Products of Influenza A Virus.Cold Spring Harbor perspectives in medicine · 2021Review
- Influenza A virus segments five and six can harbor artificial introns allowing expanded coding capacity.PLoS pathogens · 2021Article
- The Structure, Function, and Pathobiology of the Influenza A and B Virus Ion Channels.Cold Spring Harbor perspectives in medicine · 2020Review
- Influenza A Virus M2 Protein: Roles from Ingress to Egress.International journal of molecular sciences · 2017Review
- A compensatory mutagenesis study of a conserved hairpin in the M gene segment of influenza A virus shows its role in virus replication.RNA biology · 2017Article
- Computational and molecular analysis of conserved influenza A virus RNA secondary structures involved in infectious virion production.RNA biology · 2016Article
- Influenza virus mRNA trafficking through host nuclear speckles.Nature microbiology · 2016Article
- Structural Analysis of the Roles of Influenza A Virus Membrane-Associated Proteins in Assembly and Morphology.Journal of virology · 2015Article
- Adaptive mutation in influenza A virus non-structural gene is linked to host switching and induces a novel protein by alternative splicing.Emerging microbes & infections · 2012Article
- Identification of a novel splice variant form of the influenza A virus M2 ion channel with an antigenically distinct ectodomain.PLoS pathogens · 2012Article
- Article
- Influenza virus m2 ion channel protein is necessary for filamentous virion formation.Journal of virology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Influenza A virus genome RNA segment 7 encodes three known mRNAs, two of which, M2 mRNA and M mRNA3, are derived by alternative splicing of the primary collinear mRNA transcript using alternative 5' splice sites. The function of M mRNA3 is currently unknown, therefore we attempted to determine whether it is essential for virus replication. Recombinant viruses unable to produce M mRNA3 and/or M2 mRNA were created by mutating the shared 3' splice site. Growth of the mutant viruses in M2-expressing MDCK cells was not significantly affected by the lack of M mRNA3. During the course of a wild-type virus infection, levels of M mRNA3 began to decrease while those of M2 mRNA increased, which may indicate a potential mechanism of alternative splicing control. These data suggest that neither M mRNA3 nor any potential protein product are essential for influenza virus replication in tissue culture.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.