ArticleThe Journal of biological chemistry2009
Ski regulates muscle terminal differentiation by transcriptional activation of Myog in a complex with Six1 and Eya3.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Sine oculis homeobox homolog family function in gastrointestinal cancer: Progression and comprehensive analysis.World journal of clinical oncology · 2025Review
- Transcription factor roles in the local adaptation to temperature in the Andean Spiny Toad Rhinella spinulosa.Scientific reports · 2024Article
- Article
- RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis.iScience · 2023Article
- Proteome-wide profiling of transcriptional machinery on accessible chromatin with biotinylated transposons.Science advances · 2021Article
- Knockdown of Ski decreases osteosarcoma cell proliferation and migration by suppressing the PI3K/Akt signaling pathway.International journal of oncology · 2020Article
- miR-23a-3p/SIX1 regulates glucose uptake and proliferation through GLUT3 in head and neck squamous cell carcinomas.Journal of Cancer · 2020Article
- Time course and side-by-side analysis of mesodermal, pre-myogenic, myogenic and differentiated cell markers in the chicken model for skeletal muscle formation.Journal of anatomy · 2015Article
- The role of Six1 in the genesis of muscle cell and skeletal muscle development.International journal of biological sciences · 2014Review
- Overexpression of Six1 leads to retardation of myogenic differentiation in C2C12 myoblasts.Molecular biology reports · 2013Article
- Six1 and Six1 cofactor expression is altered during early skeletal muscle overload in mice.The journal of physiological sciences : JPS · 2012Article
- Chromosomal instability in mouse embryonic fibroblasts null for the transcriptional co-repressor Ski.Journal of cellular physiology · 2012Article
- Mitogen-activated protein kinase extracellular signal-regulated kinase 2 phosphorylates and promotes Pin1 protein-dependent promyelocytic leukemia protein turnover.The Journal of biological chemistry · 2011Article
- Peroxisome proliferator-activated receptor γ (PPARγ) mediates a Ski oncogene-induced shift from glycolysis to oxidative energy metabolism.The Journal of biological chemistry · 2011Article
- Excitation-transcription coupling in skeletal muscle: the molecular pathways of exercise.Biological reviews of the Cambridge Philosophical Society · 2011Review
- Characterization of microRNAs from sheep (Ovis aries) using computational and experimental analyses.Molecular biology reports · 2011Article
- Transcriptional analysis of the titin cap gene.Molecular genetics and genomics : MGG · 2011Article
- Kruppel-like factor 4 inhibits epithelial-to-mesenchymal transition through regulation of E-cadherin gene expression.The Journal of biological chemistry · 2010Article
- Promyelocytic leukemia protein controls cell migration in response to hydrogen peroxide and insulin-like growth factor-1.The Journal of biological chemistry · 2010Article
- The Notch effector Hey1 associates with myogenic target genes to repress myogenesis.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Overexpression of the Ski pro-oncogene has been shown to induce myogenesis in non-muscle cells, to promote muscle hypertrophy in postnatal mice, and to activate transcription of muscle-specific genes. However, the precise role of Ski in muscle cell differentiation and its underlying molecular mechanism are not fully understood. To elucidate the involvement of Ski in muscle terminal differentiation, two retroviral systems were used to achieve conditional overexpression or knockdown of Ski in satellite cell-derived C2C12 myoblasts. We found that enforced expression of Ski promoted differentiation, whereas loss of Ski severely impaired it. Compromised terminal differentiation in the absence of Ski was likely because of the failure to induce myogenin (Myog) and p21 despite normal expression of MyoD. Chromatin immunoprecipitation and transcriptional reporter experiments showed that Ski occupied the endogenous Myog regulatory region and activated transcription from the Myog regulatory region upon differentiation. Transactivation of Myog was largely dependent on a MEF3 site bound by Six1, not on the binding site of MyoD or MEF2. Activation of the MEF3 site required direct interaction of Ski with Six1 and Eya3 mediated by the evolutionarily conserved Dachshund homology domain of Ski. Our results indicate that Ski is necessary for muscle terminal differentiation and that it exerts this role, at least in part, through its association with Six1 and Eya3 to regulate the Myog transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.