ArticleExperimental hematology2008
Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cells.
Article in Experimental hematology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Generation of Novel Monoclonal Antibodies Recognizing Rabbit CD34 Antigen.Biomolecules · 2025Article
- Using apheresis-derived cells to augment microdrilling in the treatment of chondral defects in an ovine model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- Peripheral mononuclear blood cell apheresis in a preclinical ovine model.BMC veterinary research · 2018Article
- A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Boosting Hematopoietic Engraftment after in Utero Transplantation through Vascular Niche Manipulation.Stem cell reports · 2016Article
- Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications.Cell and tissue banking · 2016Article
- In utero stem cell transplantation and gene therapy: rationale, history, and recent advances toward clinical application.Molecular therapy. Methods & clinical development · 2016Review
- Influence of a dual-injection regimen, plerixafor and CXCR4 on in utero hematopoietic stem cell transplantation and engraftment with use of the sheep model.Cytotherapy · 2014Article
- Temporal definition of haematopoietic stem cell niches in a large animal model of in utero stem cell transplantation.British journal of haematology · 2014Article
- CD34 affinity pheresis attenuates a surge among circulating progenitor cells following vascular injury.Journal of vascular surgery · 2014Article
- In utero therapy for congenital disorders using amniotic fluid stem cells.Frontiers in pharmacology · 2014Article
- Hemophilia A: an ideal disease to correct in utero.Frontiers in pharmacology · 2014Review
- Lung progenitors from lambs can differentiate into specialized alveolar or bronchiolar epithelial cells.BMC veterinary research · 2013Article
- Treatment of Hemophilia A in Utero and Postnatally using Sheep as a Model for Cell and Gene Delivery.Journal of genetic syndromes & gene therapy · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveWe and many others have long used sheep as a predictive model system in which to explore stem cell transplantation. Unfortunately, while numerous markers are available to identify and isolate human hematopoietic stem cells (HSC), no reagents exist that allow HSC/progenitors from sheep to be identified or purified, greatly impeding the application of this well-established large animal model to the study of autologous or allogeneic HSC transplantation. The current studies were undertaken to create a monoclonal antibody to sheep CD34 that would enable isolation and study of sheep HSC/progenitors. MATERIALS AND
methodsA partial cDNA to the extracellular domain of the sheep CD34 antigen was polymerase chain reaction cloned, characterized, and used to genetically immunize mice and create hybridomas.
resultsThe resultant monoclonal antibody to sheep CD34 allows flow cytometric detection of sheep HSC/progenitors present within bone marrow, cord blood, and mobilized peripheral blood. Moreover, this antibody can be used to enrich for HSC/progenitors with enhanced in vitro colony-forming potential, and also identifies endothelial cells in situ within paraffin-embedded tissue sections, similarly to antibodies to human CD34.
conclusionsThe availability of this monoclonal antibody recognizing the stem cell antigen CD34 in sheep will greatly facilitate the study of autologous and allogeneic HSC transplantation using this clinically relevant large animal model.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.