ArticleBioTechniques2008
Improvements to bead-based oligonucleotide ligation SNP genotyping assays.
Article in BioTechniques, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- High throughput Luminex beads based multiplex assay for identification of six major bacterial pathogens of mastitis in dairy animals.Frontiers in cellular and infection microbiology · 2023Article
- Single Nucleotide Polymorphisms inDiagnostics (Basel, Switzerland) · 2022Review
- Review
- A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay.Scientific reports · 2019Article
- Validation of a microRNA target site polymorphism in H3F3B that is potentially associated with a broad schizophrenia phenotype.PloS one · 2018Article
- Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications.Mutation research. Reviews in mutation research · 2017Review
- A primerless molecular diagnostic: phosphorothioated-terminal hairpin formation and self-priming extension (PS-THSP).Analytical and bioanalytical chemistry · 2016Article
- Plasmodium falciparum parasite population structure and gene flow associated to anti-malarial drugs resistance in Cambodia.Malaria journal · 2016Article
- Efficient SNP Discovery by Combining Microarray and Lab-on-a-Chip Data for Animal Breeding and Selection.Microarrays (Basel, Switzerland) · 2015Review
- Catechol-O-methyltransferase Val158met polymorphism interacts with early experience to predict executive functions in early childhood.Developmental psychobiology · 2015Article
- A genome scan for loci shared by autism spectrum disorder and language impairment.The American journal of psychiatry · 2014Article
- Simultaneous and sensitive detection of human immunodeficiency virus type 1 (HIV) drug resistant genotypes by multiplex oligonucleotide ligation assay.Journal of virological methods · 2013Article
- RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers.BMC biotechnology · 2011Article
- Article
- A multiplex single nucleotide polymorphism typing assay for detecting mutations that result in decreased fluoroquinolone susceptibility in Salmonella enterica serovars Typhi and Paratyphi A.The Journal of antimicrobial chemotherapy · 2010Article
- Increasing genotype-phenotype model determinism: application to bivariate reading/language traits and epistatic interactions in language-impaired families.Human heredity · 2010Article
- Identification of a schizophrenia-associated functional noncoding variant in NOS1AP.The American journal of psychiatry · 2009Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We describe a bead-based, multiplexed, oligonucleotide ligation assay (OLA) performed on the Luminex flow cytometer. Differences between this method and those previously reported include the use of far fewer beads and the use of a universal oligonucleotide for signal detection. These innovations serve to significantly reduce the cost of the assay, while maintaining robustness and accuracy. Comparisons are made between the Luminex OLA and both pyrosequencing and direct sequencing. Experiments to assess conversion rates, call rates, and concordance across technical replicates are also presented.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.