ArticleJournal of cellular physiology2009
Biochemical characterization of membrane fractions in murine sperm: identification of three distinct sub-types of membrane rafts.
Article in Journal of cellular physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 73 citations in OpenAlex.
- Expression, Localization, and Processing of Chicken Sperm ADAM32L2 during the Acrosome Reaction: A Possible Function in the Sperm-Egg Interaction.The journal of poultry science · 2025Article
- Lipid remodeling in acrosome exocytosis: unraveling key players in the human sperm.Frontiers in cell and developmental biology · 2024Review
- Segmental differentiation of the murine epididymis: identification of segment-specific, GM1-enriched vesicles and regulation by luminal fluid factors†.Biology of reproduction · 2023Article
- New Insights on Sperm Function in Male Infertility of Unknown Origin: A Multimodal Approach.Biomolecules · 2023Article
- Age-Dependent Variations in Functional Quality and Proteomic Characteristics of Canine (International journal of molecular sciences · 2022Article
- Testis-Specific Isoform of NaInternational journal of molecular sciences · 2022Review
- Role of Plasmalemma Microdomains (Rafts) in Protection of the Plant Cell Under Osmotic Stress.The Journal of membrane biology · 2021Article
- Non-detergent Isolation of Membrane Structures from Beet Plasmalemma and Tonoplast Having Lipid Composition Characteristic of Rafts.The Journal of membrane biology · 2020Article
- Identification of lipid raft glycoproteins obtained from boar spermatozoa.Glycoconjugate journal · 2020Article
- Src family kinases-mediated negative regulation of sperm acrosome reaction in chickens (Gallus gallus domesticus).PloS one · 2020Article
- Testis-Specific Isoform of Na/K-ATPase (ATP1A4) Interactome in Raft and Non-Raft Membrane Fractions from Capacitated Bovine Sperm.International journal of molecular sciences · 2019Article
- Diversity of ejaculated sperm proteins in Moxotó bucks (Animal reproduction · 2018Article
- Case study on the pathophysiology of Fabry disease: abnormalities of cellular membranes can be reversed by substrate reductionBioscience reports · 2017Article
- Redistribution of soluble N-ethylmaleimide-sensitive-factor attachment protein receptors in mouse sperm membranes prior to the acrosome reaction.Biology of reproduction · 2017Article
- Characterization of the functions and proteomes associated with membrane rafts in chicken sperm.PloS one · 2017Article
- A new front in cell invasion: The invadopodial membrane.European journal of cell biology · 2016Review
- Comparison of Membrane Characteristics between Freshly Ejaculated and Cryopreserved Sperm in the Chicken.The journal of poultry science · 2016Article
- Organization of Membrane Rafts in Chicken Sperm.The journal of poultry science · 2016Article
- Ultrastructure and lipid composition of detergent-resistant membranes derived from mammalian sperm and two types of epithelial cells.Cell and tissue research · 2016Article
- Oviductosome-Sperm Membrane Interaction in Cargo Delivery: DETECTION OF FUSION AND UNDERLYING MOLECULAR PLAYERS USING THREE-DIMENSIONAL SUPER-RESOLUTION STRUCTURED ILLUMINATION MICROSCOPY (SR-SIM).The Journal of biological chemistry · 2015Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Despite enormous interest in membrane raft micro-domains, no studies in any cell type have defined the relative compositions of the raft fractions on the basis of their major components--sterols, phospholipids, and proteins--or additional raft-associating lipids such as the ganglioside, G(M1). Our previous localization data in live sperm showed that the plasma membrane overlying the acrosome represents a stabilized platform enriched in G(M1) and sterols. These findings, along with the physiological requirement for sterol efflux for sperm to function, prompted us to characterize sperm membrane fractions biochemically. After confirming limitations of commonly used detergent-based approaches, we utilized a non-detergent-based method, separating membrane fractions that were reproducibly distinct based on sterol, G(M1), phospholipid, and protein compositions (both mass amounts and molar ratios). Based on fraction buoyancy and biochemical composition, we identified at least three highly reproducible sub-types of membrane raft. Electron microscopy revealed that raft fractions were free of visible contaminants and were separated by buoyancy rather than morphology. Quantitative proteomic comparisons and fluorescence localization of lipids suggested that different organelles contributed differentially to individual raft sub-types, but that multiple membrane micro-domain sub-types could exist within individual domains. This has important implications for scaffolding functions broadly associated with rafts. Most importantly, we show that the common practice of characterizing membrane domains as either "raft" or "non-raft" oversimplifies the actual biochemical complexity of cellular membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.