ArticleMolecular biology of the cell2009
Coiled-coil-mediated dimerization is not required for myosin VI to stabilize actin during spermatid individualization in Drosophila melanogaster.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Hydrogen/Deuterium Exchange Mass Spectrometry Provides Insights into the Role of Drosophila Testis-Specific Myosin VI Light Chain AndroCaM.Biochemistry · 2024Article
- Investigations of human myosin VI targeting using optogenetically controlled cargo loading.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Gudu, an Armadillo repeat-containing protein, is required for spermatogenesis in Drosophila.Gene · 2013Article
- The cortical acto-Myosin network: from diffusion barrier to functional gateway in the transport of neurosecretory vesicles to the plasma membrane.Frontiers in endocrinology · 2013Review
- Myosin VI and its cargo adaptors - linking endocytosis and autophagy.Journal of cell science · 2013Review
- Structure of androcam supports specialized interactions with myosin VI.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Drosophila spermiogenesis: Big things come from little packages.Spermatogenesis · 2012Article
- Myosin VI in PC12 cells plays important roles in cell migration and proliferation but not in catecholamine secretion.Journal of muscle research and cell motility · 2011Article
- Myosin VI contributes to synaptic transmission and development at the Drosophila neuromuscular junction.BMC neuroscience · 2011Article
- Blanks, a nuclear siRNA/dsRNA-binding complex component, is required for Drosophila spermiogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Myosin VI regulates actin structure specialization through conserved cargo-binding domain sites.PloS one · 2011Article
- Principles of unconventional myosin function and targeting.Annual review of cell and developmental biology · 2011Review
- Myosin VI dimerization triggers an unfolding of a three-helix bundle in order to extend its reach.Molecular cell · 2009Article
- Unconventional myosins acting unconventionally.Trends in cell biology · 2009Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Myosin VI is a pointed-end-directed actin motor that is thought to function as both a transporter of cargoes and an anchor, capable of binding cellular components to actin for long periods. Dimerization via a predicted coiled coil was hypothesized to regulate activity and motor properties. However, the importance of the coiled-coil sequence has not been tested in vivo. We used myosin VI's well-defined role in actin stabilization during Drosophila spermatid individualization to test the importance in vivo of the predicted coiled coil. If myosin VI functions as a dimer, a forced dimer should fully rescue myosin VI loss of function defects, including actin stabilization, actin cone movement, and cytoplasmic exclusion by the cones. Conversely, a molecule lacking the coiled coil should not rescue at all. Surprisingly, neither prediction was correct, because each rescued partially and the molecule lacking the coiled coil functioned better than the forced dimer. In extracts, no cross-linking into higher molecular weight forms indicative of dimerization was observed. In addition, a sequence required for altering nucleotide kinetics to make myosin VI dimers processive is not required for myosin VI's actin stabilization function. We conclude that myosin VI does not need to dimerize via the predicted coiled coil to stabilize actin in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.