Evidence map›Paper›PMID 19005152›Full record

ArticleJournal of clinical microbiology2009

Genotyping of Chlamydophila psittaci by real-time PCR and high-resolution melt analysis.

Stephanie L Mitchell, Bernard J Wolff, W Lanier Thacker, Paula G Ciembor, Christopher R Gregory, Karin D E Everett, Branson W Ritchie, Jonas M Winchell

Abstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
3.0field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 62 citations in OpenAlex.

  1. Article
  2. Complete genomes of twoMicrobiology resource announcements · 2025
    Article
  3. Article
  4. Multiplex Real-time PCR Assay for the Detection of allAnnals of laboratory medicine · 2023
    Article
  5. Infectious agents present in monk parakeet (Frontiers in veterinary science · 2023
    Article
  6. Genomic analysis ofJournal of genomics · 2023
    Article
  7. Atypical pneumonia caused by Chlamydia psittaci during the COVID-19 pandemic.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2022
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Seroprevalence ofEuropean journal of microbiology & immunology · 2017
    Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Distinctive features between community-acquired pneumonia (CAP) due to Chlamydophila psittaci and CAP due to Legionella pneumophila admitted to the intensive care unit (ICU).European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012
    Article
  19. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Stephanie L MitchellRespiratory Disease Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, MS G-03, Atlanta, GA 30333, USA.
Bernard J Wolff
W Lanier Thacker
Paula G Ciembor
Christopher R Gregory
Karin D E Everett
Branson W Ritchie
Jonas M Winchell
Centers for Disease Control and Prevention · USUniversity of Georgia · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human infection with Chlamydophila (Chlamydia) psittaci can lead to psittacosis, a disease that occasionally results in severe pneumonia and other medical complications. C. psittaci is currently grouped into seven avian genotypes: A through F and E/B. Serological testing, outer membrane protein A (ompA) gene sequencing, and restriction fragment length polymorphism analysis are currently used for distinguishing these genotypes. Although accurate, these methods are time-consuming and require multiple confirmatory tests. By targeting the ompA gene, a real-time PCR assay has been developed to rapidly detect and genotype C. psittaci by light-upon-extension chemistry and high-resolution melt analysis. Using this assay, we screened 169 animal specimens; 98 were positive for C. psittaci (71.4% genotype A, 3.1% genotype B, 4.1% genotype E, and 21.4% unable to be typed). This test may provide insight into the distribution of each genotype among specific hosts and provide epidemiological and epizootiological data in human and mammalian/avian cases. This diagnostic assay may also have veterinary applications during chlamydial outbreaks, particularly with respect to identifying the sources and tracking the movements of a particular genotype when multiple animal facilities are affected.

Indexed as

Transition TemperatureAnimalsBacterial Outer Membrane ProteinsBirdsChlamydophila psittaciDNA, BacterialDNA PrimersHumansPolymerase Chain ReactionPsittacosisBacterial Outer Membrane ProteinsDNA, BacterialDNA PrimersOMPA outer membrane proteins

Identifiers

PMID19005152
PMCPMC2620869
OpenAlexW2039589648

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.