ArticleNucleic acids research2008
Depurination within the intergenic region of Brome mosaic virus RNA3 inhibits viral replication in vitro and in vivo.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- A Novel Cysteine Protease fromPlants (Basel, Switzerland) · 2025Article
- Improved pokeweed genome assembly and early gene expression changes in response to jasmonic acid.BMC plant biology · 2024Article
- Article
- The Pokeweed Leaf mRNA Transcriptome and Its Regulation by Jasmonic Acid.Frontiers in plant science · 2016Article
- Pokeweed antiviral protein: its cytotoxicity mechanism and applications in plant disease resistance.Toxins · 2015Review
- Pokeweed antiviral protein alters splicing of HIV-1 RNAs, resulting in reduced virus production.RNA (New York, N.Y.) · 2014Article
- Depurination of Brome mosaic virus RNA3 inhibits its packaging into virus particles.Nucleic acids research · 2011Article
- Recombination of 5' subgenomic RNA3a with genomic RNA3 of Brome mosaic bromovirus in vitro and in vivo.Virology · 2011Article
- Homodimerization of pokeweed antiviral protein as a mechanism to limit depurination of pokeweed ribosomes.Molecular plant pathology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pokeweed antiviral protein (PAP) is a glycosidase of plant origin that has been shown to depurinate some viral RNAs in vitro. We have demonstrated previously that treatment of Brome mosaic virus (BMV) RNAs with PAP inhibited their translation in a cell-free system and decreased their accumulation in barley protoplasts. In the current study, we map the depurination sites on BMV RNA3 and describe the mechanism by which replication of the viral RNA is inhibited by depurination. Specifically, we demonstrate that the viral replicase exhibited reduced affinity for depurinated positive-strand RNA3 compared with intact RNA3, resulting in less negative-strand product. This decrease was due to depurination within the intergenic region of RNA3, between ORF3 and 4, and distant from the 3' terminal core promoter required for initiation of negative-strand RNA synthesis. Depurination within the intergenic region alone inhibited the binding of the replicase to full-length RNA3, whereas depurination outside the intergenic region permitted the replicase to initiate negative-strand synthesis; however, elongation of the RNA product was stalled at the abasic nucleotide. These results support a role of the intergenic region in controlling negative-strand RNA synthesis and contribute new insight into the effect of depurination by PAP on BMV replication.
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