ArticleProceedings of the National Academy of Sciences of the United States of America2008
Profiling antibody responses by multiparametric analysis of primary B cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 68 citations in OpenAlex.
- Challenges and future perspectives for high-throughput chimeric antigen receptor T cell discovery.Current opinion in biotechnology · 2024Review
- Deciphering cellular complexity: advances and future directions in single-cell protein analysis.Frontiers in bioengineering and biotechnology · 2024Review
- CellCelector™ as a platform in isolating primary B cells for antibody discovery.Antibody therapeutics · 2022Article
- Measuring single-cell protein secretion in immunology: Technologies, advances, and applications.European journal of immunology · 2021Review
- Single-Cell Technologies for the Study of Antibody-Secreting Cells.Frontiers in immunology · 2021Review
- Review
- High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics.Nature biotechnology · 2020Article
- How single-cell immunology is benefiting from microfluidic technologies.Microsystems & nanoengineering · 2020Review
- Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring.Biotechnology and bioengineering · 2019Review
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- Article
- Single-cell antibody nanowells: a novel technology in detecting anti-SSA/Ro60- and anti-SSB/La autoantibody-producing cells in peripheral blood of rheumatic disease patients.Arthritis research & therapy · 2016Article
- Single-Cell Detection of Secreted Aβ and sAPPα from Human IPSC-Derived Neurons and Astrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Protein adsorption in microengraving immunoassays.Sensors (Basel, Switzerland) · 2015Article
- Stochastic particle barcoding for single-cell tracking and multiparametric analysis.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Nanowell-based immunoassays for measuring single-cell secretion: characterization of transport and surface binding.Analytical chemistry · 2014Article
- Microdevices for examining immunological responses of single cells to HIV.Bioscience reports · 2014Review
- Reconfigurable microfluidics with integrated aptasensors for monitoring intercellular communication.Lab on a chip · 2014Article
- Article
- Micropatterned photodegradable hydrogels for the sorting of microbeads and cells.Angewandte Chemie (International ed. in English) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Determining the efficacy of a vaccine generally relies on measuring neutralizing antibodies in sera. This measure cannot elucidate the mechanisms responsible for the development of immunological memory at the cellular level, however. Quantitative profiles that detail the cellular origin, extent, and diversity of the humoral (antibody-based) immune response would improve both the assessment and development of vaccines. Here, we describe a novel approach to collect multiparametric datasets that describe the specificity, isotype, and apparent affinity of the antibodies secreted from large numbers of individual primary B cells (approximately 10(3)-10(4)). The antibody/antigen binding curves obtained by this approach can be used to classify closely related populations of cells using algorithms for data clustering, and the relationships among populations can be visualized graphically using affinity heatmaps. The technique described was used to evaluate the diversity of antigen-specific antibody-secreting cells generated during an in vivo humoral response to a series of immunizations designed to mimic a multipart vaccination. Profiles correlating primary antibody-producing cells with the molecular characteristics of their secreted antibodies should facilitate both the evaluation of candidate vaccines and, broadly, studies on the repertoires of antibodies generated in response to infectious or autoimmune diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.