ArticleProceedings of the National Academy of Sciences of the United States of America2008
A single-cell view on the ecophysiology of anaerobic phototrophic bacteria.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 154 papers.
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Who cites it
154 citing papers in PubMed, 430 citations in OpenAlex.
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- Microbial phosphorus cycling in terrestrial ecosystems.Nature reviews. Microbiology · 2026Review
- Abundance-activity decoupling in sulfur-cycling bacteria reflects viral infection types in meromictic lakes.The ISME journal · 2026Article
- Restoration of deuterium marker for multi-isotope mapping of cellular metabolic activity.Scientific reports · 2026Article
- Extensive data mining uncovers novel diversity among members of the rare biosphere within the Thermoplasmatota.Microbiome · 2025Article
- Phototrophic bacteria as potential probiotics for corals.npj biodiversity · 2025Review
- Viral activity in lake analogs of anoxic early Earth oceans.Microbiome · 2025Article
- Physically stable yet biologically sensitive: multiyear ecological dynamics of anoxygenic phototrophs in stably redox-stratified Lake Cadagno.Aquatic sciences · 2025Article
- From single pioneers to complex pro- and eukaryotic microbial networks in soils along a glacier forefield chronosequence in continental Antarctica.Frontiers in microbiology · 2025Article
- Nanopore sequencing enables novel detection of deuterium incorporation in DNA.Computational and structural biotechnology journal · 2024Article
- Review
- Linking morphology, genome, and metabolic activity of uncultured magnetotactic Nitrospirota at the single-cell level.Microbiome · 2024Article
- Large scale exploration reveals rare taxa crucially shape microbial assembly in alkaline lake sediments.NPJ biofilms and microbiomes · 2024Article
- Tissue-resident bacteria in metabolic diseases: emerging evidence and challenges.Nature metabolism · 2024Review
- Single-cell analysis reveals an active and heterotrophic microbiome in the Guaymas Basin deep subsurface with significant inorganic carbon fixation by heterotrophs.Applied and environmental microbiology · 2024Article
- Species-resolved, single-cell respiration rates reveal dominance of sulfate reduction in a deep continental subsurface ecosystem.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- High microbiome and metabolome diversification in coexisting sponges with different bio-ecological traits.Communications biology · 2024Article
- Core taxa drive microeukaryotic community stability of a deep subtropical reservoir after complete mixing.Environmental microbiology reports · 2023Article
- Single cell dynamics and nitrogen transformations in the chain forming diatom Chaetoceros affinis.The ISME journal · 2023Article
- Gold-FISH enables targeted NanoSIMS analysis of plant-associated bacteria.The New phytologist · 2023Article
94 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative information on the ecophysiology of individual microorganisms is generally limited because it is difficult to assign specific metabolic activities to identified single cells. Here, we develop and apply a method, Halogen In Situ Hybridization-Secondary Ion Mass Spectroscopy (HISH-SIMS), and show that it allows simultaneous phylogenetic identification and quantitation of metabolic activities of single microbial cells in the environment. Using HISH-SIMS, individual cells of the anaerobic, phototropic bacteria Chromatium okenii, Lamprocystis purpurea, and Chlorobium clathratiforme inhabiting the oligotrophic, meromictic Lake Cadagno were analyzed with respect to H(13)CO(3)(-) and (15)NH(4)(+) assimilation. Metabolic rates were found to vary greatly between individual cells of the same species, showing that microbial populations in the environment are heterogeneous, being comprised of physiologically distinct individuals. Furthermore, C. okenii, the least abundant species representing approximately 0.3% of the total cell number, contributed more than 40% of the total uptake of ammonium and 70% of the total uptake of carbon in the system, thereby emphasizing that numerically inconspicuous microbes can play a significant role in the nitrogen and carbon cycles in the environment. By introducing this quantification method for the ecophysiological roles of individual cells, our study opens a variety of possibilities of research in environmental microbiology, especially by increasing the ability to examine the ecophysiological roles of individual cells, including those of less abundant and less active microbes, and by the capacity to track not only nitrogen and carbon but also phosphorus, sulfur, and other biological element flows within microbial communities.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.