ArticleProceedings of the National Academy of Sciences of the United States of America2008
Real-time luminescence monitoring of cell-cycle and respiratory oscillations in yeast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 66 citations in OpenAlex.
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- Capturing and Understanding the Dynamics and Heterogeneity of Gene Expression in the Living Cell.International journal of molecular sciences · 2020Review
- Eukaryotic cell biology is temporally coordinated to support the energetic demands of protein homeostasis.Nature communications · 2020Article
- A bioluminescent reporter for the halophilic archaeon Haloferax volcanii.Extremophiles : life under extreme conditions · 2020Article
- Instability of the steady state solution in cell cycle population structure models with feedback.Journal of mathematical biology · 2019Article
- Promoter-dependent nuclear RNA degradation ensures cell cycle-specific gene expression.Communications biology · 2019Article
- Flavin-based metabolic cycles are integral features of growth and division in single yeast cells.Scientific reports · 2018Article
- A minimal "push-pull" bistability model explains oscillations between quiescent and proliferative cell states.Molecular biology of the cell · 2018Article
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- Reducing hypoxia and inflammation during invasive pulmonary aspergillosis by targeting the Interleukin-1 receptor.Scientific reports · 2016Article
- Cell cycle Start is coupled to entry into the yeast metabolic cycle across diverse strains and growth rates.Molecular biology of the cell · 2016Article
- Dual-Color Monitoring Overcomes the Limitations of Single Bioluminescent Reporters in Fast-Growing Microbes and Reveals Phase-Dependent Protein Productivity during the Metabolic Rhythms of Saccharomyces cerevisiae.Applied and environmental microbiology · 2015Article
- Cell cycle dynamics: clustering is universal in negative feedback systems.Journal of mathematical biology · 2015Article
- Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy.Molecular biology of the cell · 2014Article
- Noise-induced dispersion and breakup of clusters in cell cycle dynamics.Journal of theoretical biology · 2014Article
- Cell cycle dynamics in a response/signalling feedback system with a gap.Journal of biological dynamics · 2014Article
- Visible light alters yeast metabolic rhythms by inhibiting respiration.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The use of luciferase reporters has become a precise, noninvasive, high-throughput method for real-time monitoring of promoter activity in living cells, especially for rhythmic biological processes such as circadian rhythms. We developed a destabilized firefly luciferase as a reporter for rhythmic promoter activity in both the cell division and respiratory cycles of the budding yeast Saccharomyces cerevisiae in which real-time luminescence reporters have not been previously applied. The continuous output of light from luciferase reporters allowed us to explore the relationship between the cell division cycle and the yeast respiratory oscillation, including the observation of responses to chemicals that cause phase shifting of the respiratory oscillations. Destabilized firefly luciferase is a good reporter of cell cycle position in synchronized or partially synchronized yeast cultures, in both batch and continuous cultures. In addition, the oxygen dependence of luciferase can be used under certain conditions as a genetically encodable oxygen monitor. Finally, we use this reporter to show that there is a direct correlation between premature induction of cell division and phase resetting of the respiratory oscillation under the continuous culture conditions tested.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.