ArticleThe EMBO journal1991
An acetylcholine receptor alpha-subunit promoter conferring preferential synaptic expression in muscle of transgenic mice.
Article in The EMBO journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 88 citations in OpenAlex.
- Assessment of the expression and role of the α1-nAChR subunit in efferent cholinergic function during the development of the mammalian cochlea.Journal of neurophysiology · 2016Article
- Bone marrow-derived mesenchymal stem cells up-regulate acetylcholine receptor delta subunit through NRG/ErbB3-mediated mitogen-activated protein kinase pathway.Clinical and translational science · 2012Article
- LL5beta: a regulator of postsynaptic differentiation identified in a screen for synaptically enriched transcripts at the neuromuscular junction.The Journal of cell biology · 2005Article
- Sodium channel mRNAs at the neuromuscular junction: distinct patterns of accumulation and effects of muscle activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Src, Fyn, and Yes are not required for neuromuscular synapse formation but are necessary for stabilization of agrin-induced clusters of acetylcholine receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Synapse-specific and neuregulin-induced transcription require an ets site that binds GABPalpha/GABPbeta.Genes & development · 1998Article
- Implication of a multisubunit Ets-related transcription factor in synaptic expression of the nicotinic acetylcholine receptor.The EMBO journal · 1998Article
- An acetylcholine receptor alpha subunit promoter confers intrathymic expression in transgenic mice. Implications for tolerance of a transgenic self-antigen and for autoreactivity in myasthenia gravis.The Journal of clinical investigation · 1998Article
- Branched O-linked oligosaccharides ectopically expressed in transgenic mice reduce primary T-cell immune responses.The EMBO journal · 1997Article
- Transplantation of quail collagen-tailed acetylcholinesterase molecules onto the frog neuromuscular synapse.The Journal of cell biology · 1997Article
- Accumulation in fetal muscle and localization to the neuromuscular junction of cAMP-dependent protein kinase A regulatory and catalytic subunits RI alpha and C alpha.The Journal of cell biology · 1996Article
- Adaptation of nicotinic acetylcholine receptor, myogenin, and MRF4 gene expression to long-term muscle denervation.The Journal of cell biology · 1995Article
- Identification of a DNA element determining synaptic expression of the mouse acetylcholine receptor delta-subunit gene.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- A novel synapse-associated noncoding RNA.Molecular and cellular biology · 1994Article
- Neural regulation of acetylcholinesterase mRNAs at mammalian neuromuscular synapses.The Journal of cell biology · 1994Article
- In vivo and in vitro analysis of electrical activity-dependent expression of muscle acetylcholine receptor genes using adenovirus.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Neural regulation of muscle acetylcholine receptor epsilon- and alpha-subunit gene promoters in transgenic mice.The Journal of cell biology · 1993Article
- An E box mediates activation and repression of the acetylcholine receptor delta-subunit gene during myogenesis.Molecular and cellular biology · 1993Article
- An 83-nucleotide promoter of the acetylcholine receptor epsilon-subunit gene confers preferential synaptic expression in mouse muscle.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Chicken neuronal acetylcholine receptor alpha 2-subunit gene exhibits neuron-specific expression in the brain and spinal cord of transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have obtained transgenic mice expressing nuclearly targeted beta-galactosidase (nls-beta-gal) under the control of a chicken acetylcholine receptor alpha-subunit promoter. The expression of the transgene was detected in early somites, starting before embryonic day 9.5. In 13-day embryos, the expression pattern of the transgene closely paralleled that of the endogenous mouse alpha-subunit gene, assessed by in situ hybridization. Our results illustrate, with single-cell resolution, the tissue specificity of this alpha-subunit promoter during embryogenesis. After birth, the overall beta-galactosidase activity rapidly decreased with age. However, in diaphragms of newborn animals, beta-galactosidase activity selectively persisted in nuclei underlying the motor endplates. The latter were revealed by an acetylcholinesterase stain. Nls-beta-gal was also visualized by indirect immunofluorescence, while endplates were labelled with fluorescent alpha-bungarotoxin. Confocal microscopy unambiguously identified the more intensely stained nuclei as synaptic 'fundamental nuclei', and allowed estimates of relative staining levels. Thus an 842 bp acetylcholine receptor gene promoter confers preferential synaptic expression to a reporter gene within myofibres in vivo.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.