ArticleCytotechnology2007
On-line monitoring of infected Sf-9 insect cell cultures by scanning permittivity measurements and comparison with off-line biovolume measurements.
Article in Cytotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Dual-Similarity Driven Just-in-Time Modeling Enables Precise Real-Time Monitoring of Cell Cultures via Dielectric Spectroscopy.Biotechnology journal · 2026Article
- High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cells.Applied microbiology and biotechnology · 2021Article
- Application of dielectric spectroscopy to unravel the physiological state of microorganisms: current state, prospects and limits.Applied microbiology and biotechnology · 2020Review
- Dielectric Spectroscopy and Optical Density Measurement for the Online Monitoring and Control of Recombinant Protein Production in Stably Transformed Drosophila melanogaster S2 Cells.Sensors (Basel, Switzerland) · 2018Article
- Metabolic Control in Mammalian Fed-Batch Cell Cultures for Reduced Lactic Acid Accumulation and Improved Process Robustness.Bioengineering (Basel, Switzerland) · 2016Article
- Universal Capacitance Model for Real-Time Biomass in Cell Culture.Sensors (Basel, Switzerland) · 2015Article
- Real-time monitoring of adherent Vero cell density and apoptosis in bioreactor processes.Cytotechnology · 2012Article
- Introducing process analytical technology (PAT) in filamentous cultivation process development: comparison of advanced online sensors for biomass measurement.Journal of industrial microbiology & biotechnology · 2011Article
- Evaluation of an online biomass probe to monitor cell growth and cell death.BMC proceedings · 2011Article
- On-line monitoring of responses to nutrient feed additions by multi-frequency permittivity measurements in fed-batch cultivations of CHO cells.Cytotechnology · 2010Article
- A novel approach for using dielectric spectroscopy to predict viable cell volume (VCV) in early process development.Biotechnology progressArticle
- Hands-free from inoculation to harvest: Microbial fermentation with multivariate model to automate induction of recombinant protein expression.Biotechnology progressArticle
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Authors and funding
3 authors.
Funding
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Abstract
Two infected Sf-9 cell cultures were monitored on-line by multi-frequency permittivity measurements using the Fogale BIOMASS SYSTEM((R)) and by applying different off-line methods (CASY((R))1, Vi-CELLtrade mark, packed cell volume) to measure the biovolume and the mean diameter of the cell population. During the growth phase and the early infection phase the measured permittivity at the working frequency correlated well with the different off-line methods for the biovolume. We found a value of 0.67 pF cm(-1) permittivity per unit of total biovolume (CASY) (muL mL(-1)). After the maximum value in the permittivity was reached, i.e. when the viability of the cultures decreased significantly, we observed different time courses for the biovolume depending on the applied method. The differences were compared and could be explained by the underlying measurement principles. Furthermore, the characteristic frequency (f(C)) was calculated from the on-line scanning permittivity measurements. The f(C) may provide an indication of changes in cell diameter and membrane properties especially after infection and could also be an indicator for the onset of the virus production phase. The changes in f(C) were qualitatively explained by the underlying equation that is correlating f(C) and the properties of the cell population (cell diameter, intracellular conductivity and capacitance per membrane area).
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