ArticleCytotechnology2000
Advances in animal cell recombinant protein production: GS-NS0 expression system.
Article in Cytotechnology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed.
- Combining gene amplification with SEAP-reporter to achieve high expression of recombinant omicron XBB.1.5 spike protein.Protein expression and purification · 2027Article
- Recombinant erythropoietin expression elevates by ubiquitous chromatin opening element (UCOE) in CHO DG44 cells.Biotechnology letters · 2026Article
- Incorporating shaken 24-deep-well plate fed-batch culture shortens CHO cell line development time.Cytotechnology · 2025Article
- A Comparative Review on the Production of Factor VIII in Human and Non-human Hosts.Current pharmaceutical design · 2025Review
- Review
- Modulating antibody effector functions by Fc glycoengineering.Biotechnology advances · 2023Review
- Screening Strategies for High-Yield Chinese Hamster Ovary Cell Clones.Frontiers in bioengineering and biotechnology · 2022Review
- Mammalian Cell Culture as a Platform for Veterinary Vaccines.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Rapid Purification of Immunoglobulin G Using a Protein A-immobilized Monolithic Spin Column with Hydrophilic Polymers.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- The role of magnetic field in the biopharmaceutical production: Current perspectives.Biotechnology reports (Amsterdam, Netherlands) · 2019Review
- Rapid characterization of secreted recombinant proteins by native mass spectrometry.Communications biology · 2018Article
- Improving Immunotherapy Through Glycodesign.Frontiers in immunology · 2018Review
- The enhancement of antibody concentration and achievement of high cell density CHO cell cultivation by adding nucleoside.Cytotechnology · 2017Article
- Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives.Critical reviews in biotechnology · 2016Review
- Immunogenomic engineering of a plug-and-(dis)play hybridoma platform.Nature communications · 2016Article
- Cyclin and DNA distributed cell cycle model for GS-NS0 cells.PLoS computational biology · 2015Article
- On-chip microelectrode impedance analysis of mammalian cell viability during biomanufacturing.Biomicrofluidics · 2014Article
- Over-expression of secreted proteins from mammalian cell lines.Protein science : a publication of the Protein Society · 2014Article
- Recent advances in mammalian protein production.FEBS letters · 2014Review
- Mouse cell line authentication.Cytotechnology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The production of recombinant proteins using mammalian cell expression systems is of growing importance within biotechnology, largely due to the ability of specific mammalian cells to carry out post-translational modifications of the correct fidelity. The Glutamine Synthetase-NS0 system is now one such industrially important expression system.Glutamine synthetase catalyses the formation ofglutamine from glutamate and ammonia. NS0 cellscontain extremely low levels of endogenous glutaminesynthetase activity, therefore exogenous glutaminesynthetase can be used efficiently as a selectablemarker to identify successful transfectants in theabsence of glutamine in the media. In addition, theinclusion of methionine sulphoximine, an inhibitor ofglutamine synthetase activity, enables furtherselection of those clones producing relatively highlevels of transfected glutamine synthetase and henceany heterologous gene which is coupled to it. Theglutamine synthetase system technology has been usedfor research and development purposes during thisdecade and its importance is clearly demonstrated nowthat two therapeutic products produced using thissystem have reached the market place.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.