ArticleCytotechnology2006
Efficient transfection of primary zebrafish fibroblasts by nucleofection.
Article in Cytotechnology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Emerging Frontiers in Zebrafish Embryonic and Adult-Derived Cell Lines.International journal of molecular sciences · 2025Review
- Nucleic acid direct delivery to fibroblasts: a review of nucleofection and applications.Journal of biological engineering · 2022Review
- The herbal extract deriving from aerial parts of Scutellaria baicalensis shows anti-inflammation and anti-hypoxia responses in cultured fin cells from rabbit fish.Fish & shellfish immunology · 2020Article
- Versican is crucial for the initiation of cardiovascular lumen development in medaka (Oryzias latipes).Scientific reports · 2019Article
- Gene delivery into Siberian sturgeon cell lines by commercial transfection reagents.In vitro cellular & developmental biology. Animal · 2019Article
- Culture and Transfection of Zebrafish Primary Cells.Journal of visualized experiments : JoVE · 2018Article
- Nonviral Gene Therapy for Cancer: A Review.Diseases (Basel, Switzerland) · 2018Review
- Chromosome-Encoded Hemolysin, Phospholipase, and Collagenase in Plasmidless Isolates of Photobacterium damselae subsp. damselae Contribute to Virulence for Fish.Applied and environmental microbiology · 2017Article
- High Efficiency Low Cost Fibroblast Nucleofection for GMP Compatible Cell-based Gene Therapy.International journal of medical sciences · 2017Article
- Phobalysin, a Small β-Pore-Forming Toxin of Photobacterium damselae subsp. damselae.Infection and immunity · 2015Article
- Abnormal differentiation of dopaminergic neurons in zebrafish trpm7 mutant larvae impairs development of the motor pattern.Developmental biology · 2014Article
- Deriving cell lines from zebrafish embryos and tumors.Zebrafish · 2013Article
- A method to evaluate the efficiency of transfection reagents in an adherent zebrafish cell line.BioResearch open access · 2013Article
- Enhanced transfection of cell lines from Atlantic salmon through nucoleofection and antibiotic selection.BMC research notes · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although various gene delivery techniques are available, their application in zebrafish cell cultures has not been extensively studied. Here, we report that nucleofection of zebrafish primary embryonic fibroblasts results in higher transfection efficiency in comparison to other non-viral gene delivery methods. The transfection was performed using green fluorescent protein (GFP) gene constructs of a different size. Greatest DNA uptake was obtained with 4.9-kb plasmid, resulting in 43% GFP positive cells. Nucleofection with 7.4-kb pH2B-GFP plasmid followed by geneticin (G418) selection was successfully used to establish a cell line expressing nuclear histone 2B-GFP fusion protein. Efficient transfection of zebrafish fibroblasts by nucleofection offers a non-viral technique of plasmid delivery and can be used to overexpress genes of interest in these cells.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.