ArticleCytotechnology2006
Automated time-lapse microscopy and high-resolution tracking of cell migration.
Article in Cytotechnology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Deriving the A/B Cells Policy as a Robust Multi-Object Cell Pipeline for Time-Lapse Microscopy.International journal of molecular sciences · 2025Article
- QSOX1 Modulates Glioblastoma Cell Proliferation and Migration In Vitro and Invasion In Vivo.Cancers · 2024Article
- Viewing early life without labels: optical approaches for imaging the early embryo†.Biology of reproduction · 2024Review
- Exosomal L1CAM Stimulates Glioblastoma Cell Motility, Proliferation, and Invasiveness.International journal of molecular sciences · 2019Article
- A simple and accurate rule-based modeling framework for simulation of autocrine/paracrine stimulation of glioblastoma cell motility and proliferation by L1CAM in 2-D culture.BMC systems biology · 2017Article
- Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice.Journal of visualized experiments : JoVE · 2016Article
- Small-molecule inhibitors of FGFR, integrins and FAK selectively decrease L1CAM-stimulated glioblastoma cell motility and proliferation.Cellular oncology (Dordrecht, Netherlands) · 2016Article
- The agar diffusion scratch assay--A novel method to assess the bioactive and cytotoxic potential of new materials and compounds.Scientific reports · 2016Article
- Genome-wide screening identified that miR-134 acts as a metastasis suppressor by targeting integrin β1 in hepatocellular carcinoma.PloS one · 2014Article
- A review of spatial computational models for multi-cellular systems, with regard to intestinal crypts and colorectal cancer development.Journal of mathematical biology · 2013Review
- L1CAM stimulates glioma cell motility and proliferation through the fibroblast growth factor receptor.Clinical & experimental metastasis · 2013Article
- Photostable fluorescent organic dots with aggregation-induced emission (AIE dots) for noninvasive long-term cell tracing.Scientific reports · 2013Article
- L1 stimulation of human glioma cell motility correlates with FAK activation.Journal of neuro-oncology · 2011Article
- Article
- Soluble L1CAM promotes breast cancer cell adhesion and migration in vitro, but not invasion.Cancer cell international · 2010Article
- Stimulation of glioma cell motility by expression, proteolysis, and release of the L1 neural cell recognition molecule.Cancer cell international · 2009Article
- 3D multi-cell simulation of tumor growth and angiogenesis.PloS one · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from closely spaced time points (minutes), (2) over long periods (hours to days), (3) from multiple areas of interest, (4) in parallel under several different experimental conditions. Quantitation of true individual and average cell velocity and path length was obtained with high spatial and temporal resolution in "scratch" or "wound healing" assays. This revealed unique motility dynamics of drug-treated and adhesion molecule-transfected cells and, thus, this is a considerable improvement over current methods of measurement and analysis. Several fluorescent vital labeling methods commonly used for end-point analyses (GFP expression, DiO lipophilic dye, and Qtracker nanocrystals) were found to be useful for time-lapse studies under specific conditions that are described. To illustrate one application, fluorescently labeled tumor cells were seeded onto cell monolayers expressing ectopic adhesion molecules, and this resulted in consistently reduced tumor cell migration velocities. These highly quantitative time-lapse analysis methods will promote the creation of new cell motility assays and increase the resolution and accuracy of existing assays.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.