ArticleCytotechnology2008
Identification and verification of rodent cell lines by polymerase chain reaction.
Article in Cytotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
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- Study Models forMicroorganisms · 2025Review
- Identification of Iranian BHK-21-C5 Cell Line by Two Steps Polymerase Chain Reaction.Archives of Razi Institute · 2021Article
- Generation of interspecies mouse-rat chimeric embryos by embryonic stem (ES) cell microinjection.STAR protocols · 2021Article
- Long read sequencing reveals poxvirus evolution through rapid homogenization of gene arrays.eLife · 2018Article
- Simple and fast multiplex PCR method for detection of species origin in meat products.Journal of food science and technology · 2018Article
- Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking.Cell and tissue banking · 2017Article
- Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model.Journal of visualized experiments : JoVE · 2015Article
- Mouse cell line authentication.Cytotechnology · 2014Article
- Epigenetic upregulation of HGF and c-Met drives metastasis in hepatocellular carcinoma.PloS one · 2013Article
- The necessity of identity assessment of animal intestinal cell lines: A case report.Cytotechnology · 2012Article
- Species identification and authentification of human and rodent cell cultures using polymerase chain reaction analysis of vomeronasal receptor genes.Cytotechnology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell lines represent valuable tools for basic research and diagnostic applications as well as for the production of biological products such as antibodies or vaccines. For all cell culturists, a well-identified origin of their cell lines as well as the periodic re-examination of their identity should be a basic requirement. We established a simple polymerase chain reaction (PCR) to verify or identify rodent and human cell lines. Since mouse-, rat-, Chinese hamster- and Syrian hamster-derived cell lines represent the most frequently used rodent cell lines, our investigations were focused on these species. Our assay used oligonucleotide primers annealing to sequences within the beta-actin and the beta-globin gene and to repetitive DNA. Primers were designed mostly from intron sequences of the genes aiming to amplify only one specific DNA segment and thus enabling to exclude easily false DNA. More than 130 cells lines originating from the five species were analyzed in that study. Our PCR revealed specific profiles for all species investigated. No further methods like DNA sequencing or fragment length polymorphism analysis were needed to differentiate these species. The results introduce our PCR-assay as a rapid, specific and routinely feasible tool in order to identify or distinguish rodent cell lines from each other and from human cell lines.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.