ArticleCytotechnology2000
Three heterotrimeric laminins produced by human keratinocytes.
Article in Cytotechnology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed, 3 citations in OpenAlex.
- Bioactive modification of poly(ethylene glycol) hydrogels for tissue engineering.Biomaterials · 2010Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laminins are a family of glycoproteins composed of alpha,beta and gamma chains. Five alpha(alpha1-alpha5), three beta (beta1-beta3) and twogamma (gamma1 and gamma2) chains have been cloned fromhuman and their replaceable assembly into heterotrimers producesthe variety of laminins. Reverse transcription-polymerase chainreaction of mRNAs showed that human keratinocytes express thealpha3, alpha5, beta1, beta3, gamma1 andgamma2 genes at high level among the ten cloned lamininchains. Western blot and immunoprecipitation of the cell lysatewith antiserum directed against mouse laminin-1(alpha1beta1gamma1) detected two trimers with thecomposition of alphaxbeta1gamma1 (probablylaminin-10 with the composition of alpha5beta1gamma1and alphaybeta1gamma1. Meanwhile, antiserum directedagainst a synthetic peptide of human alpha3 detected onlyalpha3beta3gamma2 trimer (laminin-5). We thus show thatkeratinocytes produce three heterotrimeric laminins. We couldnot detect the assembly of alpha3 with beta1 and gamma1chains to form alpha3beta1gamma1 (laminin-6) in keratinocytes.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.