ArticlePlant molecular biology2009
Arabidopsis microtubule-associated protein AtMAP65-2 acts as a microtubule stabilizer.
Article in Plant molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Review
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- The Potential Role ofPlants (Basel, Switzerland) · 2024Article
- Review
- Comprehensive analyses of microtubule-associated protein MAP65 family genes in Cucurbitaceae and CsaMAP65s expression profiles in cucumber.Journal of applied genetics · 2023Article
- Heat stress reveals a specialized variant of the pachytene checkpoint in meiosis of Arabidopsis thaliana.The Plant cell · 2022Article
- Proteins immunologically related to MAP65-1 accumulate and localize differentially during bud development in Vitis vinifera L.Protoplasma · 2017Article
- Genome-wide analysis of the TPX2 family proteins in Eucalyptus grandis.BMC genomics · 2016Article
- Multifunctional Microtubule-Associated Proteins in Plants.Frontiers in plant science · 2016Review
- Stochastic Optical Reconstruction Microscopy Imaging of Microtubule Arrays in Intact Arabidopsis thaliana Seedling Roots.Scientific reports · 2015Article
- Preprophase band formation and cortical division zone establishment: RanGAP behaves differently from microtubules during their band formation.Plant signaling & behavior · 2015Article
- MAPs: cellular navigators for microtubule array orientations in Arabidopsis.Plant cell reports · 2014Review
- Unravelling the proteomic profile of rice meiocytes during early meiosis.Frontiers in plant science · 2014Article
- Lessons from in vitro reconstitution analyses of plant microtubule-associated proteins.Frontiers in plant science · 2014Review
- Light-regulated hypocotyl elongation involves proteasome-dependent degradation of the microtubule regulatory protein WDL3 in Arabidopsis.The Plant cell · 2013Article
- Arabidopsis microtubule-associated protein MAP65-3 cross-links antiparallel microtubules toward their plus ends in the phragmoplast via its distinct C-terminal microtubule binding domain.The Plant cell · 2012Article
- Microtubule-associated proteins MAP65-1 and MAP65-2 positively regulate axial cell growth in etiolated Arabidopsis hypocotyls.The Plant cell · 2011Article
- Arabidopsis thaliana MAP65-1 and MAP65-2 function redundantly with MAP65-3/PLEIADE in cytokinesis downstream of MPK4.Plant signaling & behavior · 2011Article
- Tobacco microtubule-associated protein, MAP65-1c, bundles and stabilizes microtubules.Plant molecular biology · 2010Article
- Arabidopsis kinetochore fiber-associated MAP65-4 cross-links microtubules and promotes microtubule bundle elongation.The Plant cell · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nine genes that encode proteins of the MAP65 family have been identified in the Arabidopsis thaliana genome. In this study, we reported that AtMAP65-2, a member of the AtMAP65 family, could strongly stabilize microtubules (MTs). Bacterially-expressed AtMAP65-2 fusion proteins induced the formation of large MT bundles in vitro. Although AtMAP65-2 showed little effect on MT assembly or nucleation, AtMAP65-2 greatly stabilized MTs that were subjected to low-temperature treatment in vitro. Analyses of truncated versions of AtMAP65-2 indicated that the region that encompassed amino acids 495-578, which formed a flexible extended loop, played a crucial role in the stabilization of MTs. Analysis of suspension-cultured Arabidopsis cells that expressed the AtMAP65-2-GFP fusion protein showed that AtMAP65-2 co-localized with MTs throughout the cell cycle. Cortical MTs that were decorated with AtMAP65-2-GFP were more resistant to the MT-disrupting drug propyzamide and to ice treatment in vivo. The results of this study demonstrate that AtMAP65-2 strongly stabilizes MTs and is involved in the regulation of MT organization and dynamics.
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