Evidence map›Paper›PMID 18997344›Full record

ArticleActa crystallographica. Section F, Structural biology and crystallization communications2008

Purification, crystallization and X-ray diffraction analysis of pavine N-methyltransferase from Thalictrum flavum.

Ankur Jain, Jörg Ziegler, David K Liscombe, Peter J Facchini, Paul A Tucker, Santosh Panjikar

Open access · bronzeAbstract read
In one paragraph

Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

Ankur JainEMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany.
Jörg Ziegler
David K Liscombe
Peter J Facchini
Paul A Tucker
Santosh Panjikar
University of Calgary · CAEuropean Molecular Biology Laboratory · DEIndian Institute of Technology Roorkee · IN

Funding

Medical Research Council G0500367
6 · The paper itself

Abstract

A cDNA from the plant Thalictrum flavum encoding pavine N-methyltransferase, an enzyme belonging to a novel class of S-adenosylmethionine-dependent N-methyltransferases specific for benzylisoquinoline alkaloids, has been heterologously expressed in Escherichia coli. The enzyme was purified using affinity and gel-filtration chromatography and was crystallized in space group P2(1). The structure was solved at 2.0 A resolution using a xenon derivative and the single isomorphous replacement with anomalous scattering method.

Indexed as

Cloning, MolecularCrystallizationMethyltransferasesMolecular Sequence DataPlant ProteinsRecombinant ProteinsThalictrumX-Ray DiffractionMethyltransferasesPlant ProteinsRecombinant Proteins

Identifiers

PMID18997344
PMCPMC2581683
OpenAlexW2066086125

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.