ArticleApplied and environmental microbiology2009
Overproduction of exopolysaccharides by an Escherichia coli K-12 rpoS mutant in response to osmotic stress.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
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- Diversification of Lipopeptide Analogues Drives Versatility in Biological Activities.Journal of agricultural and food chemistry · 2025Article
- A single rare σ70 variant establishes a unique gene expression pattern in the E. coli pathobiont LF82.Nucleic acids research · 2024Article
- A computational model for lipid-anchored polysaccharide export by the outer membrane protein GfcD.Biophysical journal · 2024Article
- Characterization of Exopolysaccharides Having Potential Antiviral Properties from Priestia Aryabattai Strain MK1 and Bacillus sp. Strain MK2.Current microbiology · 2024Article
- Mechanosensitive channel MscS is critical for termination of the bacterial hypoosmotic permeability response.The Journal of general physiology · 2023Article
- Tyrosine phosphorylation controlled poly(A) polymerase I activity regulates general stress response in bacteria.Life science alliance · 2023Article
- Disinfecting Action of Gaseous Ozone on OXA-48-ProducingInternational journal of environmental research and public health · 2022Article
- Predatory Bacteria Select for Sustained Prey Diversity.Microorganisms · 2021Article
- Exopolysaccharide anchoring creates an extreme resistance to sedimentation.Journal of bacteriology · 2021Article
- Heat-responsive and time-resolved transcriptome and metabolome analyses of Escherichia coli uncover thermo-tolerant mechanisms.Scientific reports · 2020Article
- Efficacy of A Poly(MeOEGMA) Brush on the Prevention ofAntibiotics (Basel, Switzerland) · 2020Article
- Two genomic regions encoding exopolysaccharide production systems have complementary functions in B. cereus multicellularity and host interaction.Scientific reports · 2020Article
- Variant O89 O-Antigen ofFrontiers in microbiology · 2018Article
- Article
- Article
- Transcriptional Responses of Escherichia coli to a Small-Molecule Inhibitor of LolCDE, an Essential Component of the Lipoprotein Transport Pathway.Journal of bacteriology · 2016Article
- Effects of Lipopolysaccharide Core Sugar Deficiency on Colanic Acid Biosynthesis in Escherichia coli.Journal of bacteriology · 2016Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yjbEFGH operon is implicated in the production of an exopolysaccharide of an unknown function and is induced by osmotic stress and negatively regulated by the general stress response sigma factor RpoS. Despite the obvious importance of RpoS, negative selection for rpoS has been reported to take place in starved cultures, suggesting an adaptive occurrence allowing the overexpression of RpoD-dependent uptake and nutrient-scavenging systems. The trade-off of the RpoS-dependent functions for improved nutrient utilization abilities makes the bacterium more sensitive to environmental stressors, e.g., osmotic stress. In this work, we addressed the hypothesis that overinduction of genes in rpoS-deficient strains indicates their essentiality. Using DNA microarrays, real-time PCR, and transcriptional fusions, we show that genes of the wca operon, implicated in the production of the colanic acid exopolysaccharide, previously shown to be induced by osmotic stress, are also negatively controlled by RpoS. Both exopolysaccharides in the synthesis of which yjb and wca are involved are overproduced in an rpoS mutant during osmotic stress. We also show that both operons are essential in an rpoS-deficient strain but not in the wild type; promoters of both operons are constitutively active in yjb rpoS mutants; this strain produces extremely mucoid colonies, forms long filaments, and exhibits a reduced growth capability. In addition, the wca rpoS mutant's growth is inhibited by osmotic stress. These results indicate that although induced in the wild type, both operons are much more valuable for an rpoS-deficient strain, suggesting that the overproduction of both exopolysaccharides is an adaptive action.
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