ArticleNucleic acids research2008
Efficient processing of TFO-directed psoralen DNA interstrand crosslinks by the UvrABC nuclease.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- UvrA expression ofJournal of food science and technology · 2017Article
- Review
- Removal of misincorporated ribonucleotides from prokaryotic genomes: an unexpected role for nucleotide excision repair.PLoS genetics · 2013Article
- Review
- Replication-fork stalling and processing at a single psoralen interstrand crosslink in Xenopus egg extracts.PloS one · 2011Article
- Targeting and processing of site-specific DNA interstrand crosslinks.Environmental and molecular mutagenesis · 2010Review
- Mammalian nucleotide excision repair proteins and interstrand crosslink repair.Environmental and molecular mutagenesis · 2010Review
- DNA interstrand crosslink repair in mammalian cells: step by step.Critical reviews in biochemistry and molecular biology · 2010Review
- Targeted gene conversion induced by triplex-directed psoralen interstrand crosslinks in mammalian cells.Nucleic acids research · 2009Article
- Mismatch repair and nucleotide excision repair proteins cooperate in the recognition of DNA interstrand crosslinks.Nucleic acids research · 2009Article
- The yin and yang of repair mechanisms in DNA structure-induced genetic instability.Mutation researchReview
- Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Photoreactive psoralens can form interstrand crosslinks (ICLs) in double-stranded DNA. In eubacteria, the endonuclease UvrABC plays a key role in processing psoralen ICLs. Psoralen-modified triplex-forming oligonucleotides (TFOs) can be used to direct ICLs to specific genomic sites. Previous studies of pyrimidine-rich methoxypsoralen-modified TFOs indicated that the TFO inhibits cleavage by UvrABC. Because different chemistries may alter the processing of TFO-directed ICLs, we investigated the effect of another type of triplex formed by purine-rich TFOs on the processing of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) ICLs by the UvrABC nuclease. Using an HMT-modified TFO to direct ICLs to a specific site, we found that UvrABC made incisions on the purine-rich strand of the duplex approximately 3 bases from the 3'-side and approximately 9 bases from the 5'-side of the ICL, within the TFO-binding region. In contrast to previous reports, the UvrABC nuclease cleaved the TFO-directed psoralen ICL with a greater efficiency than that of the psoralen ICL alone. Furthermore, the TFO was dissociated from its duplex binding site by UvrA and UvrB. As mutagenesis by TFO-directed ICLs requires nucleotide excision repair, the efficient processing of these lesions supports the use of triplex technology to direct DNA damage for genome modification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.