ArticleNeuroscience2009
Human olfactory epithelial cells generated in vitro express diverse neuronal characteristics.
Article in Neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 56 citations in OpenAlex.
- Olfactory models as a platform for neuroscience research.Frontiers in cellular neuroscience · 2026Review
- Altered PLCβ/IPBiomedicines · 2024Article
- Biomarkers in Alzheimer's Disease: Are Olfactory Neuronal Precursors Useful forBrain sciences · 2024Review
- Potential of olfactory neuroepithelial cells as a model to study schizophrenia: A focus on GPCRs (Review).International journal of molecular medicine · 2024Review
- Unraveling the Link between Olfactory Deficits and Neuropsychiatric Disorders.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Review
- Coronavirus infection in chemosensory cells.Journal of neurovirology · 2023Article
- Antidepressant Low Doses of Ketamine and Melatonin in Combination Produce Additive Neurogenesis in Human Olfactory Neuronal Precursors.Molecules (Basel, Switzerland) · 2022Article
- Optimization of Neurite Tracing and Further Characterization of Human Monocyte-Derived-Neuronal-like Cells.Brain sciences · 2021Article
- Altered Secretome and ROS Production in Olfactory Mucosa Stem Cells Derived from Friedreich's Ataxia Patients.International journal of molecular sciences · 2020Article
- Zika virus infection in chemosensory cells.Journal of neurovirology · 2020Article
- Potential Use of Exfoliated and Cultured Olfactory Neuronal Precursors for In Vivo Alzheimer's Disease Diagnosis: A Pilot Study.Cellular and molecular neurobiology · 2020Article
- Cannabis Users Show Enhanced Expression of CBMolecular neurobiology · 2018Article
- Transdifferentiation of Human Circulating Monocytes Into Neuronal-Like Cells in 20 Days and Without Reprograming.Frontiers in molecular neuroscience · 2018Article
- Variant in a common odorant-binding protein gene is associated with bitter sensitivity in people.Behavioural brain research · 2017Article
- Rat models of spinal cord injury: from pathology to potential therapies.Disease models & mechanisms · 2016Review
- Office-based olfactory mucosa biopsies.International forum of allergy & rhinology · 2016Article
- Altered G Protein Coupling in Olfactory Neuroepithelial Cells From Patients With Schizophrenia.Schizophrenia bulletin · 2016Article
- Translational potential of olfactory mucosa for the study of neuropsychiatric illness.Translational psychiatry · 2015Review
- Oxidative stress and schizophrenia: recent breakthroughs from an old story.Current opinion in psychiatry · 2014Review
- Olfactory cells via nasal biopsy reflect the developing brain in gene expression profiles: utility and limitation of the surrogate tissues in research for brain disorders.Neuroscience research · 2013Article
Corrections and comments
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Authors and funding
14 authors at 8 institutions in 2 countries.
Funding
Abstract
The olfactory epithelium constitutes the sole source of regenerating neural cells that can be obtained from a living human. As such, primary cultures derived from human olfactory epithelial biopsies can be utilized to study neurobiological characteristics of individuals under different conditions and disease states. Here, using such human cultures, we report in vitro generation of cells that exhibit a complex neuronal phenotype, encompassing receptors and signaling pathways pertinent to both olfaction and other aspects of CNS function. Using in situ hybridization, we demonstrate for the first time the native expression of olfactory receptors in cultured cells derived from human olfactory epithelial tissue. We further establish the presence and function of olfactory transduction molecules in these cells using immunocytochemistry, calcium imaging and molecular methods. Western blot analysis revealed the expression of neurotransmitter receptors for dopamine (D2R), 5-HT (5HT2C) and NMDA subtypes 1 and 2A/2B. Stimulation with dopamine or 5-HT enhanced receptor G protein activation in a subtype specific manner, based on 35S-guanosine triphosphate incorporation assay. Functional characteristics of the cultured cells are demonstrated through enhanced tyrosine phosphorylation of NMDAR 2A/2B and recruitment of signaling partners in response to NMDA stimulation. The array of neuronal characteristics observed here establishes that proliferating cells derived from the human olfactory epithelium differentiate in vitro to express functional and molecular attributes of mature olfactory neurons. These cultured neural cells exhibit neurotransmitter pathways important in a number of neuropsychiatric disorders. Their ready availability from living humans thus provides a new tool to link functional and molecular features of neural cells with clinical characteristics of individual living patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.