ArticleMolecular cell2008
Organization of an activator-bound RNA polymerase holoenzyme.
Article in Molecular cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Analysis and comparison of the bacterial σ54 regulon: Evidence of phylogenetic trends in gene regulation.PloS one · 2025Article
- Anaerobic benzene oxidation inApplied and environmental microbiology · 2024Article
- Characterizing a Propionate Sensor inACS synthetic biology · 2023Article
- SPARC: Structural properties associated with residue constraints.Computational and structural biotechnology journal · 2022Article
- Bacterial Enhancer Binding Proteins-AAABiomolecules · 2020Review
- Mechanisms of σJournal of molecular biology · 2019Review
- Targeting the alternative sigma factor RpoN to combat virulence in Pseudomonas aeruginosa.Scientific reports · 2017Article
- Structures of RNA Polymerase Closed and Intermediate Complexes Reveal Mechanisms of DNA Opening and Transcription Initiation.Molecular cell · 2017Article
- Novel DNA Binding and Regulatory Activities for σJournal of bacteriology · 2017Article
- Kinetics of transcription initiation directed by multiple cis-regulatory elements on the glnAp2 promoter.Nucleic acids research · 2016Article
- Role of the σJournal of molecular biology · 2016Article
- The bacterial enhancer-dependent RNA polymerase.The Biochemical journal · 2016Review
- Structure, Regulation, and Inhibition of the Quorum-Sensing Signal Integrator LuxO.PLoS biology · 2016Article
- Mutations in RNA Polymerase Bridge Helix and Switch Regions Affect Active-Site Networks and Transcript-Assisted Hydrolysis.Journal of molecular biology · 2015Article
- Genome wide interactions of wild-type and activator bypass forms of σ54.Nucleic acids research · 2015Article
- TRANSCRIPTION. Structures of the RNA polymerase-σ54 reveal new and conserved regulatory strategies.Science (New York, N.Y.) · 2015Article
- A perspective on the enhancer dependent bacterial RNA polymerase.Biomolecules · 2015Review
- Unique ATPase site architecture triggers cis-mediated synchronized ATP binding in heptameric AAA+-ATPase domain of flagellar regulatory protein FlrC.The Journal of biological chemistry · 2015Article
- Molecular basis of nucleotide-dependent substrate engagement and remodeling by an AAA+ activator.Nucleic acids research · 2014Article
- Domain movements of the enhancer-dependent sigma factor drive DNA delivery into the RNA polymerase active site: insights from single molecule studies.Nucleic acids research · 2014Article
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Authors and funding
7 authors.
Funding
Abstract
Transcription initiation involves the conversion from closed promoter complexes, comprising RNA polymerase (RNAP) and double-stranded promoter DNA, to open complexes, in which the enzyme is able to access the DNA template in a single-stranded form. The complex between bacterial RNAP and its major variant sigma factor sigma(54) remains as a closed complex until ATP hydrolysis-dependent remodeling by activator proteins occurs. This remodeling facilitates DNA melting and allows the transition to the open complex. Here we present cryoelectron microscopy reconstructions of bacterial RNAP in complex with sigma(54) alone, and of RNAP-sigma(54) with an AAA+ activator. Together with photo-crosslinking data that establish the location of promoter DNA within the complexes, we explain why the RNAP-sigma(54) closed complex is unable to access the DNA template and propose how the structural changes induced by activator binding can initiate conformational changes that ultimately result in formation of the open complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.