ArticleAnalytical biochemistry2009
Liquid chromatography-mass spectrometry to study chondroitin lyase action pattern.
Article in Analytical biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Fabrication of natural enzyme-covered / amino-modified Pd-Pt bimetallic-doped zeolitic imidazolate framework for ultrasensitive detection of metabolites.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- Efficient Expression and Characterization of an Endo-Type Lyase HCLase_M28 and Its Gradual Scale-Up Fermentation for the Preparation of Chondroitin Sulfate Oligosaccharides.Applied biochemistry and biotechnology · 2024Article
- Heparan sulfates from bat and human lung and their binding to the spike protein of SARS-CoV-2 virus.Carbohydrate polymers · 2021Article
- Cloning and Expression of Recombinant Chondroitinase ACII and Its Comparison to the Arthrobacter aurescens Enzyme.Biotechnology journal · 2017Article
- Purification and sequence characterization of chondroitin sulfate and dermatan sulfate from fishes.Glycoconjugate journal · 2017Article
- Analysis of glycosaminoglycan-derived, precolumn, 2-aminoacridone-labeled disaccharides with LC-fluorescence and LC-MS detection.Nature protocols · 2014Article
- Development of biodegradable methylprednisolone microparticles for treatment of articular pathology using a spray-drying technique.International journal of nanomedicine · 2013Article
- Exploiting enzyme specificities in digestions of chondroitin sulfates A and C: production of well-defined hexasaccharides.Glycobiology · 2012Article
- Analysis of glycosaminoglycans in stem cell glycomics.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Glycosaminoglycans of the porcine central nervous system.Biochemistry · 2010Article
- High-performance liquid chromatography-mass spectrometry for mapping and sequencing glycosaminoglycan-derived oligosaccharides.Nature protocols · 2010Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
Liquid chromatography-mass spectrometry was applied to determine the action pattern of different chondroitin lyases. Two commercial enzymes, chondroitinase ABC (Proteus vulgaris) and chondroitinase ACII (Arthrobacter aurescens), having action patterns previously determined by viscosimetry and gel electrophoresis were first examined. Next, the action patterns of recombinant lyases, chondroitinase ABC from Bacteroides thetaiotaomicron (expressed in Escherichia coli) and chondroitinase AC from Flavobacterium heparinum (expressed in its original host), were examined. Chondroitin sulfate A (CS-A, also known as chondroitin-4-sulfate) was used as the substrate for these four lyases. Aliquots taken at various time points were analyzed. The products of chondroitinase ABC (P. vulgaris) and chondroitinase AC (F. heparinum) contained unsaturated oligosaccharides of sizes ranging from disaccharide to decasaccharide, demonstrating that both are endolytic enzymes. The products afforded by chondroitinase ABC (B. thetaiotaomicron) and chondroitinase ACII (A. aurescens) contained primarily unsaturated disaccharide. These two exolytic enzymes showed different minor products, suggesting some subtle specificity differences between the actions of these two exolytic lyases on chondroitin sulfate A.
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Registered trials
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