ArticleVirchows Archiv. A, Pathological anatomy and histopathology1991
Three-dimensional studies of the cytoskeleton of cultured hepatocytes: a quick-freezing and deep-etching study.
Article in Virchows Archiv. A, Pathological anatomy and histopathology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 28 citations in OpenAlex.
- Extraskeletal myxoid chondrosarcoma: a study using a quick-freezing and deep-etching method.Medical molecular morphology · 2009Article
- Proteoglycans in articular cartilage revealed with a quick freezing and deep etching method.Annals of the rheumatic diseases · 1996Article
- An ultrastructural study of goblet cells in rat nasal mucosa as revealed by the quick-freezing method.Journal of anatomy · 1996Article
- Different organization of intermediate filaments in columnar cells of rat large intestinal mucosa as revealed by confocal laser scanning microscopy and quick-freezing and deep-etching method.Virchows Archiv : an international journal of pathology · 1995Article
- Hepatocyte-matrix interactions.Gut · 1994Review
- Ultrastructural study of mast cells stimulated with compound 48/80 as revealed by quick-freezing method.Virchows Archiv : an international journal of pathology · 1994Article
- Immunocytochemical study of membrane skeletons in abnormally shaped erythrocytes as revealed by a quick-freezing and deep-etching method.Virchows Archiv. A, Pathological anatomy and histopathology · 1993Article
- Localization of laminin in nephritic glomeruli as revealed by a quick-freezing and deep-etching method with immunohistochemistry.The Histochemical journal · 1992Article
- Immunocytochemical study of dystrophin in cultured mouse muscle cells by the quick-freezing and deep-etching method.The Histochemical journal · 1992Article
- An ultrastructural study of the cytoplasmic aspects of erythrocyte membranes by a quick-freezing and deep-etching method.Journal of anatomy · 1992Article
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2 authors at 1 institution in 1 country.
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Abstract
The ultrastructure of the cytoskeleton of cultured mouse hepatocytes was studied by a quick-freezing and deep-etching method. Isolated mouse hepatocytes were cultured on collagen gels for 48 h, fixed in paraformaldehyde and centrifuged to prepare cell pellets. The hepatocytes were split open to remove cytoplasmic soluble proteins for replica preparations. Some specimens were decorated with anti-actin antibody or S1 myosin fragments to identify actin filaments. They were quickly frozen in isopentane-propane mixture, fractured in liquid nitrogen, deeply etched in a freeze-fracture machine and rotary shadowed by platinum and carbon. The basal cell membranes of hepatocytes were in contact with the collagen gels and the apical surface faced the culture medium. Networks of actin filaments were attached to the apical cell membranes, but intermediate filaments were localized along the basal layer. Some intermediate filaments were associated with cell organelles, such as the endoplasmic reticulum. The Golgi apparatus was less associated with the cytoskeleton and showed synthesized materials in the cisternae. Cytoskeletal organization in cultured hepatocytes was revealed three-dimensionally, indicating that the interaction of cell membranes with collagen gels is important for the organization of the cytoskeleton.
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