ArticleDigestive diseases and sciences2009
Expression of alkaline sphingomyelinase in yeast cells and anti-inflammatory effects of the expressed enzyme in a rat colitis model.
Article in Digestive diseases and sciences, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Alkaline sphingomyelinase (ENPP7) attenuates DSS-induced colitis by modulating FOXO1-mediated antioxidative stress responses.BMC gastroenterology · 2026Article
- From Classical to Alternative Pathways of 2-Arachidonoylglycerol Synthesis: AlterAGs at the Crossroad of Endocannabinoid and Lysophospholipid Signaling.Molecules (Basel, Switzerland) · 2024Review
- Alkaline sphingomyelinase deficiency impairs intestinal mucosal barrier integrity and reduces antioxidant capacity in dextran sulfate sodium-induced colitis.World journal of gastroenterology · 2024Article
- Transcriptome analysis of intestine from alk-SMase knockout mice reveals the effect of alk-SMase.Cancer cell international · 2022Article
- Transcriptome analysis alk-SMase knockout mice reveals the effect of alkaline sphingomyelinase on liver.Biochemistry and biophysics reports · 2022Article
- Alkaline sphingomyelinase (NPP7) impacts the homeostasis of intestinal T lymphocyte populations.Frontiers in immunology · 2022Article
- Breast Milk Lipids and Fatty Acids in Regulating Neonatal Intestinal Development and Protecting against Intestinal Injury.Nutrients · 2020Review
- Deficiency of alkaline SMase enhances dextran sulfate sodium-induced colitis in mice with upregulation of autotaxin.Journal of lipid research · 2018Article
- Alkaline sphingomyelinase (NPP7) in hepatobiliary diseases: A field that needs to be closely studied.World journal of hepatology · 2018Review
- A lipidomics investigation into the intervention of celastrol in experimental colitis.Molecular bioSystems · 2016Article
- Fostering Inflammatory Bowel Disease: Sphingolipid Strategies to Join Forces.Mediators of inflammation · 2016Review
- PPARα-dependent exacerbation of experimental colitis by the hypolipidemic drug fenofibrate.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
- Changes of activity and isoforms of alkaline sphingomyelinase (nucleotide pyrophosphatase phosphodiesterase 7) in bile from patients undergoing endoscopic retrograde cholangiopancreatography.BMC gastroenterology · 2014Article
- Crucial role of alkaline sphingomyelinase in sphingomyelin digestion: a study on enzyme knockout mice.Journal of lipid research · 2011Article
- A metaproteomic approach to study human-microbial ecosystems at the mucosal luminal interface.PloS one · 2011Article
- Generating ceramide from sphingomyelin by alkaline sphingomyelinase in the gut enhances sphingomyelin-induced inhibition of cholesterol uptake in Caco-2 cells.Digestive diseases and sciences · 2010Article
- Sphingolipids as Mediators in the Crosstalk between Microbiota and Intestinal Cells: Implications for Inflammatory Bowel Disease.Mediators of inflammationReview
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alkaline sphingomyelinase (Alk-SMase) is a key enzyme in the intestinal tract for digestion of dietary sphingomyelin (SM), which generates lipid messengers with cell-cycle regulating effects. The enzyme is significantly decreased in ulcerative colitis and colon cancer. Based on this information, we wanted to investigate whether the enzyme had preventive effects against murine colitis. We report herein a method to express a biologically active Alk-SMase from Pichia pastoris yeast cells. By using the expressed enzyme to treat a rat colitis model induced by dextran sulfate sodium, we found that intrarectal instillation of Alk-SMase once daily for 1 week significantly reduced the inflammation score and protected the colonic epithelium from inflammatory destruction. We found a tendency for decreased tumor necrosis factor (TNF)-alpha expression in the Alk-SMase-treated group. This study, for the first time, provides a method to produce the enzyme and shows the potential applicability of the enzyme in the treatment of inflammatory bowel diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.