ArticleMolecular vision2008
Preferential gene expression in the limbus of the vervet monkey.
Article in Molecular vision, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Wnt activation as a potential therapeutic approach to treat partial limbal stem cell deficiency.Scientific reports · 2023Article
- Differentially Expressed Tear Proteins in Sjögren's Syndrome Keratoconjunctivitis Sicca.Translational vision science & technology · 2023Article
- Regulation of Limbal Epithelial Stem Cells: Importance of the Niche.International journal of molecular sciences · 2021Review
- Human limbal epithelial stem cell regulation, bioengineering and function.Progress in retinal and eye research · 2021Review
- Wnt6 plays a complex role in maintaining human limbal stem/progenitor cells.Scientific reports · 2021Article
- Molecular characteristics and spatial distribution of adult human corneal cell subtypes.Scientific reports · 2021Article
- Limbal stem cell diseases.Experimental eye research · 2021Review
- Chondroitin Sulfate as a Potential Modulator of the Stem Cell Niche in Cornea.Frontiers in cell and developmental biology · 2020Article
- An Eye on the Wnt Inhibitory Factor Wif1.Frontiers in cell and developmental biology · 2018Review
- Frizzled 7 maintains the undifferentiated state of human limbal stem/progenitor cells.Stem cells (Dayton, Ohio) · 2014Article
- Presence of native limbal stromal cells increases the expansion efficiency of limbal stem/progenitor cells in culture.Experimental eye research · 2013Article
- Transmembrane protein PERP is a component of tessellate junctions and of other junctional and non-junctional plasma membrane regions in diverse epithelial and epithelium-derived cells.Cell and tissue research · 2013Article
- Preferential biological processes in the human limbus by differential gene profiling.PloS one · 2013Article
- Extracellular Matrix is an Important Component of Limbal Stem Cell Niche.Journal of functional biomaterials · 2012Article
- Stem cells and corneal epithelial maintenance: insights from the mouse and other animal models.Results and problems in cell differentiation · 2012Review
- Wnt/β-catenin signaling regulates proliferation of human cornea epithelial stem/progenitor cells.Investigative ophthalmology & visual science · 2011Article
- Validation of endogenous control genes for gene expression studies on human ocular surface epithelium.PloS one · 2011Article
- A comparison of stem cell-related gene expression in the progenitor-rich limbal epithelium and the differentiating central corneal epithelium.Molecular vision · 2011Article
- Keratin 13 is a more specific marker of conjunctival epithelium than keratin 19.Molecular vision · 2011Article
- Comparative transcriptional profiling of the limbal epithelial crypt demonstrates its putative stem cell niche characteristics.BMC genomics · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo elucidate the unique molecular factors and biological processes that are differentially expressed in the limbal stem cell microenvironment by comparing directly to that of its immediate adjacent structures, the cornea and conjunctiva.
methodsTotal RNA was isolated and amplified from the limbus, cornea, and conjunctiva. A gene expression profile of each tissue type was obtained by using microarray technique. The transcripts in which the expression level was at least twofold higher than that in the other two tissue types were identified. The expression levels of selected genes were confirmed by quantitative reverse transcription polymerase chain reaction (QRT-PCR). Protein expression of selected genes were confirmed by an immunohistochemistry study in normal human ocular tissue.
resultsThere were 186 preferentially overexpressed transcripts in the limbus in direct comparison to that in the cornea and conjunctiva. Many signature genes in the cornea and conjunctiva were among the preferentially expressed transcripts obtained by the microarray data. In addition, a significant number of new genes were identified, and the expression level of all nine selected genes was verified by QRT-PCR. Protein expression levels of keratin 13, tenascin c, homeodomain only protein (HOP), and TP53 apoptosis effector (PERP) were confirmed in human ocular tissues. Functional analysis of the preferentially expressed genes in the limbus reviewed that melanin metabolism and cell-cell adhesion were among the noticeable biological processes. Chromosomal distribution of the overexpressed genes in the limbus was disproportional to that of all known human genes.
conclusionsThese findings may shed light on the unique molecular components and regulation of limbal stem cells and their niche.
Indexed as
Identifiers
18989386PMC2579939W2163922876What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.