ArticleJournal of acquired immune deficiency syndromes (1999)2008
Early HIV-1 diagnosis using in-house real-time PCR amplification on dried blood spots for infants in remote and resource-limited settings.
Article in Journal of acquired immune deficiency syndromes (1999), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Randomized noninferiority trial of two maternal single-dose nevirapine-sparing regimens to prevent perinatal HIV in Thailand.AIDS (London, England) · 2015Trial
- Review
- A culture-free biphasic approach for sensitive and rapid detection of pathogens in dried whole-blood matrix.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Achieve Research Continuity During Social Distancing by Rapidly Implementing Individual and Group Videoconferencing with Participants: Key Considerations, Best Practices, and Protocols.AIDS and behavior · 2020Article
- Perinatal Antiretroviral Intensification to Prevent Intrapartum HIV Transmission When Antenatal Antiretroviral Therapy Is Initiated Less Than 8 Weeks Before Delivery.Journal of acquired immune deficiency syndromes (1999) · 2020Article
- HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system.PloS one · 2020Article
- The stability of HIV-1 nucleic acid in whole blood and improved detection of HIV-1 in alternative specimen types when compared to Dried Blood Spot (DBS) specimens.Journal of virological methods · 2018Article
- Killing of Latently HIV-Infected CD4 T Cells by Autologous CD8 T Cells Is Modulated by Nef.Frontiers in immunology · 2018Article
- Country of Birth of Children With Diagnosed HIV Infection in the United States, 2008-2014.Journal of acquired immune deficiency syndromes (1999) · 2018Article
- Miniaturized devices for point of care molecular detection of HIV.Lab on a chip · 2017Review
- Early infant HIV diagnosis and entry to HIV care cascade in Thailand: an observational study.The lancet. HIV · 2016Observational
- Uptake of early infant diagnosis in Thailand's national program for preventing mother-to-child HIV transmission and linkage to care, 2008-2011.Journal of the International AIDS Society · 2016Article
- Community-Based HIV-1 Early Diagnosis and Risk Behavior Analysis of Men Having Sex with Men in Hong Kong.PloS one · 2015Article
- The impact of a HIV prevention of mother to child transmission program in a nigerian early infant diagnosis centre.Nigerian medical journal : journal of the Nigeria Medical Association · 2014Article
- Article
- Article
- RNA versus DNA (NucliSENS EasyQ HIV-1 v1.2 versus Amplicor HIV-1 DNA test v1.5) for early diagnosis of HIV-1 infection in infants in Senegal.Journal of clinical microbiology · 2011Article
- 12-month mortality and loss-to-program in antiretroviral-treated children: The IeDEA pediatric West African Database to evaluate AIDS (pWADA), 2000-2008.BMC public health · 2011Article
- Predictors of successful early infant diagnosis of HIV in a rural district hospital in Zambézia, Mozambique.Journal of acquired immune deficiency syndromes (1999) · 2011Article
- Long-term survival of HIV-infected children receiving antiretroviral therapy in Thailand: a 5-year observational cohort study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2010Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundIn resource-limited settings, most perinatally HIV-1-infected infants do not receive timely antiretroviral therapy because early HIV-1 diagnosis is not available or affordable.
objectiveTo assess the performance of a low-cost in-house real-time polymerase chain reaction (PCR) assay to detect HIV-1 DNA in infant dried blood spots (DBS).
methodsOne thousand three hundred nineteen DBS collected throughout Thailand from non-breast-fed infants born to HIV-1-infected mothers were shipped at room temperature to a central laboratory.In-house real-time DNA PCR results were compared with Roche Amplicor HIV-1 DNA test (Version 1.5) results. In addition, we verified the Roche test performance on DBS sampled from 1218 other infants using as reference HIV serology result at 18 months of age.
resultsReal-time DNA PCR and Roche DNA PCR results were 100% concordant. Compared with HIV serology results, the Roche test sensitivity was 98.6% (95% confidence interval: 92.6% to 100.0%) and its specificity at 4 months of age was 99.7% (95% confidence interval: 99.2% to 99.9%).
conclusionsIn-house real-time PCR performed as well as the Roche test in detecting HIV-1 DNA on DBS in Thailand. Combined use of DBS and real-time PCR assays is a reliable and affordable tool to expand access to early HIV-1 diagnosis in remote and resource-limited settings, enabling timely treatment for HIV-1-infected infants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.