ArticleProceedings of the National Academy of Sciences of the United States of America2008
Visualizing the dynamics of viral replication in living cells via Tat peptide delivery of nuclease-resistant molecular beacons.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Fluorogenic Aptamer-Based Hybridization Chain Reaction for Signal-Amplified Imaging of Apurinic/Apyrimidinic Endonuclease 1 in Living Cells.Biosensors · 2024Article
- Review
- Review
- Recent Advances in the Molecular Beacon Technology for Live-Cell Single-Molecule Imaging.iScience · 2020Review
- Optimizing peptide nucleic acid probes for hybridization-based detection and identification of bacterial pathogens.The Analyst · 2019Article
- Comparing Agent-Based Delivery of DNA and PNA Forced Intercalation (FIT) Probes for Multicolor mRNA Imaging.Chembiochem : a European journal of chemical biology · 2019Article
- A CRISPR/molecular beacon hybrid system for live-cell genomic imaging.Nucleic acids research · 2018Article
- Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication.Scientific reports · 2017Article
- Novel single-cell technologies in acute myeloid leukemia research.Translational research : the journal of laboratory and clinical medicine · 2017Review
- Fluorinated molecular beacons as functional DNA nanomolecules for cellular imaging.Chemical science · 2017Article
- Engineering Novel Molecular Beacon Constructs to Study Intracellular RNA Dynamics and Localization.Genomics, proteomics & bioinformatics · 2017Review
- A molecular beacon-based approach for live-cell imaging of RNA transcripts with minimal target engineering at the single-molecule level.Scientific reports · 2017Article
- Single cell biology beyond the era of antibodies: relevance, challenges, and promises in biomedical research.Cellular and molecular life sciences : CMLS · 2017Review
- Induced Apoptosis Investigation in Wild-type and FLT3-ITD Acute Myeloid Leukemia Cells by Nanochannel Electroporation and Single-cell qRT-PCR.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- The Antimicrobial and Antiviral Applications of Cell-Penetrating Peptides.Methods in molecular biology (Clifton, N.J.) · 2015Review
- Article
- Use of flow cytometry for rapid, quantitative detection of poliovirus-infected cells via TAT peptide-delivered molecular beacons.Applied and environmental microbiology · 2013Article
- Simultaneous detection of infectious human echoviruses and adenoviruses by an in situ nuclease-resistant molecular beacon-based assay.Applied and environmental microbiology · 2012Article
- Methods to detect infectious human enteric viruses in environmental water samples.International journal of hygiene and environmental health · 2011Review
- Detection of murine norovirus-1 by using TAT peptide-delivered molecular beacons.Applied and environmental microbiology · 2011Article
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we describe the use of nuclease-resistant molecular beacons (MBs) for the real-time detection of coxsackievirus B6 replication in living Buffalo green monkey kidney (BGMK) cells via Tat peptide delivery. A nuclease-resistant MB containing 2'-O-methyl RNA bases with phosphorothioate internucleotide linkages was designed to specifically target an 18-bp 5' noncoding region of the viral genome. For intracellular delivery, a cell-penetrating Tat peptide was conjugated to the MB by using a thiol-maleimide linkage. Presence of the Tat peptide enabled nearly 100% intracellular delivery within 15 min. When the conjugate was introduced into BGMK cell monolayers infected with coxsackievirus B6, a discernible fluorescence was observed at 30 min after infection, and as few as 1 infectious viral particle could be detected within 2 h. The stability and the intracellular delivery properties of the modified MBs enabled real-time monitoring of the cell-to-cell spreading of viral infection. These results suggest that the Tat-modified, nuclease-resistant MBs may be powerful tools for improving our understanding of the dynamic behavior of viral replication and for therapeutic studies of antiviral treatments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.