ArticleNucleic acids research2009
A sequence-independent in vitro transposon-based strategy for efficient cloning of genomes of large DNA viruses as bacterial artificial chromosomes.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Complete Genome Sequence and Construction of an Infectious Bacterial Artificial Chromosome Clone of a Virulent Duck Enteritis Virus Strain XJ.Transboundary and emerging diseases · 2024Article
- The emerging role of recombineering in microbiology.Engineering microbiology · 2023Review
- Cross-species microbial genome transfer: a Review.Frontiers in bioengineering and biotechnology · 2023Review
- Direct cloning of a herpesvirus genome for rapid generation of infectious BAC clones.Journal of advanced research · 2023Article
- Highly Efficient CRISPR/Cas9-Mediated Homologous Recombination Promotes the Rapid Generation of Bacterial Artificial Chromosomes of Pseudorabies Virus.Frontiers in microbiology · 2016Article
- Human cytomegalovirus: bacterial artificial chromosome (BAC) cloning and genetic manipulation.Current protocols in microbiology · 2012Article
- Review
- Bacterial artificial chromosome clones of viruses comprising the towne cytomegalovirus vaccine.Journal of biomedicine & biotechnology · 2012Article
- Viruses and human cancer: from detection to causality.Cancer letters · 2011Review
- Recent advances in cloning herpesviral genomes as infectious bacterial artificial chromosomes.Cell cycle (Georgetown, Tex.) · 2011Review
- Cloning whole bacterial genomes in yeast.Nucleic acids research · 2010Article
- Sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens.The Journal of general virology · 2010Article
- Autoexcision of bacterial artificial chromosome facilitated by terminal repeat-mediated homologous recombination: a novel approach for generating traceless genetic mutants of herpesviruses.Journal of virology · 2010Article
- Red-mediated transposition and final release of the mini-F vector of a cloned infectious herpesvirus genome.PloS one · 2009Article
- Progress and prospects: human artificial chromosomes.Gene therapy · 2009Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Bacterial artificial chromosomes (BACs) derived from genomes of large DNA viruses are powerful tools for functional delineation of viral genes. Current methods for cloning the genomes of large DNA viruses as BACs require prior knowledge of the viral sequences or the cloning of viral DNA fragments, and are tedious because of the laborious process of multiple plaque purifications, which is not feasible for some fastidious viruses. Here, we describe a novel method for cloning the genomes of large DNA viruses as BACs, which entails direct in vitro transposition of viral genomes with a BAC cassette, and subsequent recovery in Escherichia coli. Determination of insertion sites and adjacent viral sequences identify the BAC clones for genetic manipulation and functional characterization. Compared to existing methods, this new approach is highly efficient, and does not require any information on viral sequences or cloning of viral DNA fragments, and plaque purifications. This method could potentially be used for discovering previously unidentified viruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.