ArticleMethods in molecular biology (Clifton, N.J.)2009
Flexi vector cloning.
Article in Methods in molecular biology (Clifton, N.J.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
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- Toxoplasma ceramide synthases: Gene duplication, functional divergence, and roles in parasite fitness.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- A simple and sensitive detection of the binding ligands by using the receptor aggregation and NMR spectroscopy: a test case of the maltose binding protein.Journal of biomolecular NMR · 2021Article
- Function and solution structure of the Arabidopsis thaliana RALF8 peptide.Protein science : a publication of the Protein Society · 2019Article
- Conserved Lipid and Small-Molecule Modulation of COQ8 Reveals Regulation of the Ancient Kinase-like UbiB Family.Cell chemical biology · 2018Article
- Multi-omic Mitoprotease Profiling Defines a Role for Oct1p in Coenzyme Q Production.Molecular cell · 2017Article
- Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling.Biotechnology for biofuels · 2017Article
- Relationship between recombinant protein expression and host metabolome as determined by two-dimensional NMR spectroscopy.PloS one · 2017Article
- Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.Journal of structural and functional genomics · 2015Article
- Active site and laminarin binding in glycoside hydrolase family 55.The Journal of biological chemistry · 2015Article
- Proteins interacting with cloning scars: a source of false positive protein-protein interactions.Scientific reports · 2015Article
- Use of Nanostructure-Initiator Mass Spectrometry to Deduce Selectivity of Reaction in Glycoside Hydrolases.Frontiers in bioengineering and biotechnology · 2015Article
- IRDL cloning: a one-tube, zero-background, easy-to-use, directional cloning method improves throughput in recombinant DNA preparation.PloS one · 2014Article
- Article
- Structural and functional characterization of CalS11, a TDP-rhamnose 3'-O-methyltransferase involved in calicheamicin biosynthesis.ACS chemical biology · 2013Article
- Crystal structure of an eIF4G-like protein from Danio rerio.Proteins · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
A protocol for ligation-dependent cloning using the Flexi Vector method in a 96-well format is described. The complete protocol includes PCR amplification of the desired gene to append Flexi Vector cloning sequences, restriction digestion of the PCR products, ligation of the digested PCR products into a similarly digested acceptor vector, transformation and growth of host cells, analysis of the transformed clones, and storage of a sequence-verified clone. The protocol also includes transfer of the sequence-verified clones into another Flexi Vector plasmid backbone. Smaller numbers of cloning reactions can be undertaken by appropriate scaling of the indicated reaction volumes.
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Registered trials
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