ArticleMolecular and cellular biology1991
Molecular cloning and functional analysis of three subunits of yeast proteasome.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 94 citations in OpenAlex.
- The GET pathway is a major bottleneck for maintaining proteostasis in Saccharomyces cerevisiae.Scientific reports · 2023Article
- Yeast PI31 inhibits the proteasome by a direct multisite mechanism.Nature structural & molecular biology · 2022Article
- Proteasome Control of [URE3] Prion Propagation by Degradation of Anti-Prion Proteins Cur1 and Btn2 in Saccharomyces cerevisiae.Genetics · 2021Article
- A suite of polymerase chain reaction-based peptide tagging plasmids for epitope-targeted enzymatic functionalization of yeast proteins.Yeast (Chichester, England) · 2020Article
- Article
- Proteasome subunit α1 overexpression preferentially drives canonical proteasome biogenesis and enhances stress tolerance in yeast.Scientific reports · 2019Article
- Assembly of an Evolutionarily Conserved Alternative Proteasome Isoform in Human Cells.Cell reports · 2016Article
- Genome-scale analyses of butanol tolerance in Saccharomyces cerevisiae reveal an essential role of protein degradation.Biotechnology for biofuels · 2013Article
- Article
- Plasticity in eucaryotic 20S proteasome ring assembly revealed by a subunit deletion in yeast.The EMBO journal · 2004Article
- Article
- Yeast cycloheximide-resistant crl mutants are proteasome mutants defective in protein degradation.Molecular biology of the cell · 1997Article
- Subunit arrangement in the human 20S proteasome.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Plant 21D7 protein, a nuclear antigen associated with cell division, is a component of the 26S proteasome.Plant physiology · 1997Article
- Roles of proteasomes in cell growth.Molecular biology reports · 1995Review
- Proteasomes of the yeast S. cerevisiae: genes, structure and functions.Molecular biology reports · 1995Review
- Phylogenic relationships of the amino acid sequences of prosome (proteasome, MCP) subunits.Molecular & general genetics : MGG · 1994Article
- Displacement of housekeeping proteasome subunits by MHC-encoded LMPs: a newly discovered mechanism for modulating the multicatalytic proteinase complex.The EMBO journal · 1994Article
- Mutations in PRG1, a yeast proteasome-related gene, cause defects in nuclear division and are suppressed by deletion of a mitotic cyclin gene.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- An Arabidopsis gene homologous to mammalian and insect genes encoding the largest proteasome subunit.Molecular & general genetics : MGG · 1993Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genes encoding three subunits of Saccharomyces cerevisiae proteasome were cloned and sequenced. The deduced amino acid sequences were homologous not only to each other (30 to 40% identity) but also to those of rat and Drosophila proteasomes (25 to 65% identity). However, none of these sequences showed any similarity to any other known sequences, including various proteases, suggesting that these proteasome subunits may constitute a unique gene family. Gene disruption analyses revealed that two of the three subunits (subunits Y7 and Y8) are essential for growth, indicating that the proteasome and its individual subunits play an indispensable role in fundamental biological processes. On the other hand, subunit Y13 is not essential; haploid cells with a disrupted Y13 gene can proliferate, although the doubling time is longer than that of cells with nondisrupted genes. In addition, biochemical analysis revealed that proteasome prepared from the Y13 disrupted cells contains tryptic and chymotryptic activities equivalent to those of nondisrupted cells, indicating that the Y13 subunit is not essential for tryptic or chymotryptic activity. However, the chymotryptic activity of the Y13 disrupted cells is not dependent on sodium dodecyl sulfate (SDS), an activator of proteasome, since nearly full activity was observed in the absence of SDS. Thus, the activity in proteasome of the Y13 disrupted cells might result in unregulated intracellular proteolysis, thus leading to the prolonged cell cycle. These results indicate that cloned proteasome subunits having similar sequences to the yeast Y13 subunit are structural, but not catalytic, components of proteasome. It is also suggested that two subunits (Y7 and Y8) might occupy positions essential to proteasome structure or activity, whereas subunit Y13 is in a nonessential but important position.
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