ArticleMolecular biology of the cell2009
Drosophila Orc6 facilitates GTPase activity and filament formation of the septin complex.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- Unexpected molecular mechanism of Orc6-based Meier-Gorlin syndrome: insights from a humanized Drosophila model.Genetics · 2025Article
- Septins regulate border cell surface geometry, shape, and motility downstream of Rho in Drosophila.Developmental cell · 2023Article
- Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells.The Journal of cell biology · 2023Article
- Evolutionary degeneration of septins into pseudoGTPases: impacts on a hetero-oligomeric assembly interface.Frontiers in cell and developmental biology · 2023Article
- Article
- HumanizedGenetics · 2020Article
- Structural basis of DNA replication origin recognition by human Orc6 protein binding with DNA.Nucleic acids research · 2020Article
- Phosphorylation of Pnut in the Early Stages ofG3 (Bethesda, Md.) · 2018Article
- Nonreplicative functions of the origin recognition complex.Nucleus (Austin, Tex.) · 2018Review
- The Role of Pnut and its Functional Domains inRussian journal of genetics. Applied research · 2017Article
- The Arf activator GBF1 localizes to plasma membrane sites involved in cell adhesion and motility.Cellular logistics · 2017Article
- ORC proteins in the mammalian zygote.Cell and tissue research · 2016Review
- Drosophila model of Meier-Gorlin syndrome based on the mutation in a conserved C-Terminal domain of Orc6.American journal of medical genetics. Part A · 2015Article
- Septin Form and Function at the Cell Cortex.The Journal of biological chemistry · 2015Review
- Functional insight into the role of Orc6 in septin complex filament formation in Drosophila.Molecular biology of the cell · 2015Article
- Septin functions in organ system physiology and pathology.Biological chemistry · 2014Review
- Article
- The origin recognition complex: a biochemical and structural view.Sub-cellular biochemistry · 2012Review
- Assembly of the human origin recognition complex occurs through independent nuclear localization of its components.The Journal of biological chemistry · 2011Article
- A vertebrate N-end rule degron reveals that Orc6 is required in mitosis for daughter cell abscission.The Journal of cell biology · 2011Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The origin recognition complex or ORC is a six-subunit protein important for DNA replication and other cell functions. Orc6, the smallest subunit of ORC, is essential for both replication and cytokinesis in Drosophila, and interacts with the septin protein Pnut, which is part of the Drosophila septin complex. In this study, we describe the analysis of the interaction of Orc6 with Pnut and whole Drosophila septin complex. Septin complex was purified from Drosophila embryos and also reconstituted from recombinant proteins. The interaction of Orc6 with the septin complex is dependent on the coiled-coil domain of Pnut. Furthermore, the binding of Orc6 to Pnut increases the intrinsic GTPase activity of the Drosophila septin complex, whereas in the absence of GTP it enhances septin complex filament formation. These results suggest an active role for Orc6 in septin complex function. Orc6 might be a part of a control mechanism directing the cytokinesis machinery during the final steps of mitosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.