ArticlePlant physiology2009
The genetic basis of constitutive and herbivore-induced ESP-independent nitrile formation in Arabidopsis.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 144 citations in OpenAlex.
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- The Multifaceted Health Benefits of Broccoli-A Review of Glucosinolates, Phenolics and Antimicrobial Peptides.Molecules (Basel, Switzerland) · 2025Review
- Unravelling Glucoraphanin and Glucoerucin Metabolism across Broccoli Sprout Development: Insights from Metabolite and Transcriptome Analysis.Plants (Basel, Switzerland) · 2024Article
- Nitrile-Specific Protein NSP2 and Its Interacting Protein MPK3 Synergistically Regulate Plant Disease Resistance inPlants (Basel, Switzerland) · 2023Article
- Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways.Plant communications · 2023Review
- Article
- iTRAQ-Based Proteomic Analyses of Regulation of Isothiocyanate and Endogenous Selenium Metabolism in Broccoli Sprouts by Exogenous Sodium Selenite.Foods (Basel, Switzerland) · 2023Article
- Genome-Wide Identification and Expression Analysis of ESPs and NSPs Involved in Glucosinolate Hydrolysis and Insect Attack Defense in Chinese Cabbage (Plants (Basel, Switzerland) · 2023Article
- Glucosinolates, a natural chemical arsenal: More to tell than the myrosinase story.Frontiers in microbiology · 2023Review
- The Impact of Nitrile-Specifier Proteins on Indolic Carbinol and Nitrile Formation in Homogenates ofMolecules (Basel, Switzerland) · 2022Article
- Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing ofLife (Basel, Switzerland) · 2022Article
- β-Cyanoalanine synthase protects mites against Arabidopsis defenses.Plant physiology · 2022Article
- Assessment of Methodological Pipelines for the Determination of Isothiocyanates Derived from Natural Sources.Antioxidants (Basel, Switzerland) · 2022Review
- cDNA Transcriptome ofInternational journal of molecular sciences · 2022Article
- Sulforaphane and Its Bifunctional Analogs: Synthesis and Biological Activity.Molecules (Basel, Switzerland) · 2022Review
- Nitrilases NIT1/2/3 Positively Regulate Flowering by InhibitingFrontiers in plant science · 2022Article
- Transcriptomic Reprograming ofPlants (Basel, Switzerland) · 2021Article
- So Much for Glucosinolates: A Generalist Does Survive and Develop on Brassicas, but at What Cost?Plants (Basel, Switzerland) · 2021Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glucosinolates are a group of thioglucosides that are components of an activated chemical defense found in the Brassicales. Plant tissue damage results in hydrolysis of glucosinolates by endogenous thioglucosidases known as myrosinases. Spontaneous rearrangement of the aglucone yields reactive isothiocyanates that are toxic to many organisms. In the presence of specifier proteins, alternative products, namely epithionitriles, simple nitriles, and thiocyanates with different biological activities, are formed at the expense of isothiocyanates. Recently, simple nitriles were recognized to serve distinct functions in plant-insect interactions. Here, we show that simple nitrile formation in Arabidopsis (Arabidopsis thaliana) ecotype Columbia-0 rosette leaves increases in response to herbivory and that this increase is independent of the known epithiospecifier protein (ESP). We combined phylogenetic analysis, a screen of Arabidopsis mutants, recombinant protein characterization, and expression quantitative trait locus mapping to identify a gene encoding a nitrile-specifier protein (NSP) responsible for constitutive and herbivore-induced simple nitrile formation in Columbia-0 rosette leaves. AtNSP1 is one of five Arabidopsis ESP homologues that promote simple nitrile, but not epithionitrile or thiocyanate, formation. Four of these homologues possess one or two lectin-like jacalin domains, which share a common ancestry with the jacalin domains of the putative Arabidopsis myrosinase-binding proteins MBP1 and MBP2. A sixth ESP homologue lacked specifier activity and likely represents the ancestor of the gene family with a different biochemical function. By illuminating the genetic and biochemical bases of simple nitrile formation, our study provides new insights into the evolution of metabolic diversity in a complex plant defense system.
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