ArticleNucleic acids research2008
Orientation-dependent interaction between Drosophila insulators is a property of this class of regulatory elements.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
61 citing papers in PubMed, 103 citations in OpenAlex.
- Insulators As Dynamic, Tunable Regulators of Enhancer-Promoter Coordination in LivingbioRxiv : the preprint server for biology · 2026Article
- Shared binding sites for the chromosomal architectural protein Su(Hw) mediate physical interactions betweenbioRxiv : the preprint server for biology · 2026Article
- Chromatin insulators homie and nhomie can interact with distant copies either together or separately, with distinct outcomes for enhancer-promoter interactions.PLoS genetics · 2026Article
- Configuring the Code: Enhancer-Promoter Arrangement and Transcriptional Regulation.Journal of molecular biology · 2026Review
- The homie insulator has sub-elements with different insulating and long-range pairing properties.Genetics · 2025Article
- ThebioRxiv : the preprint server for biology · 2025Article
- Chromosome structure ineLife · 2024Article
- Article
- NF-κB factors cooperate with Su(Hw)/E4F1 to balance Drosophila/human immune responses via modulating dynamic expression of miR-210.Nucleic acids research · 2024Article
- Boundary bypass activity in theOpen biology · 2023Article
- Transcriptional Readthrough Interrupts Boundary Function in Drosophila.International journal of molecular sciences · 2023Article
- Boundary Bypass Activity in thebioRxiv : the preprint server for biology · 2023Article
- Transcriptional read through interrupts boundary function in Drosophila.bioRxiv : the preprint server for biology · 2023Article
- Topological screen identifies hundreds of Cp190- and CTCF-dependentScience advances · 2023Article
- Mechanisms of Interaction between Enhancers and Promoters in ThreeInternational journal of molecular sciences · 2023Review
- Essential role of Cp190 in physical and regulatory boundary formation.Science advances · 2022Article
- Boundaries potentiate polycomb response element-mediated silencing.BMC biology · 2021Article
- Structural basis of diversity and homodimerization specificity of zinc-finger-associated domains in Drosophila.Nucleic acids research · 2021Article
- Mechanisms of Enhancer-Promoter Interactions in Higher Eukaryotes.International journal of molecular sciences · 2021Review
- Advances in Chromatin Imaging at Kilobase-Scale Resolution.Trends in genetics : TIG · 2020Review
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Insulators are defined as a class of regulatory elements that delimit independent transcriptional domains within eukaryotic genomes. According to previous data, an interaction (pairing) between some Drosophila insulators can support distant activation of a promoter by an enhancer. Here, we have demonstrated that pairs of well-studied insulators such as scs-scs, scs'-scs', 1A2-1A2 and Wari-Wari support distant activation of the white promoter by the yeast GAL4 activator in an orientation-dependent manner. The same is true for the efficiency of the enhancer that stimulates white expression in the eyes. In all insulator pairs tested, stimulation of the white gene was stronger when insulators were inserted between the eye enhancer or GAL4 and the white promoter in opposite orientations relative to each other. As shown previously, Zw5, Su(Hw) and dCTCF proteins are required for the functioning of different insulators that do not interact with each other. Here, strong functional interactions have been revealed between DNA fragments containing binding sites for either Zw5 or Su(Hw) or dCTCF protein but not between heterologous binding sites [Zw5-Su(Hw), dCTCF-Su(Hw), or dCTCF-Zw5]. These results suggest that insulator proteins can support selective interactions between distant regulatory elements.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.