ReviewPhilosophical transactions of the Royal Society of London. Series B, Biological sciences2009
Review. Peering into an ATPase ion pump with single-channel recordings.
Review in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 52 citations in OpenAlex.
- Current Flow in Nerves and Mitochondria: An Electro-Osmotic Approach.Biomolecules · 2025Review
- The Critical Role of Spreading Depolarizations in Early Brain Injury: Consensus and Contention.Neurocritical care · 2022Review
- Improved Hodgkin-Huxley type model for neural action potentials.European biophysics journal : EBJ · 2021Article
- Neural shutdown under stress: an evolutionary perspective on spreading depolarization.Journal of neurophysiology · 2020Review
- The KdpFABC complex - KMolecular membrane biology · 2019Article
- Brainstem neurons survive the identical ischemic stress that kills higher neurons: insight to the persistent vegetative state.PloS one · 2014Article
- A hyperpolarization-activated ion current of amphibian oocytes.Pflugers Archiv : European journal of physiology · 2013Article
- Article
- On the concept of resting potential--pumping ratio of the Na⁺/K⁺ pump and concentration ratios of potassium ions outside and inside the cell to sodium ions inside and outside the cell.The Journal of membrane biology · 2013Article
- Mechanism of proton/substrate coupling in the heptahelical lysosomal transporter cystinosin.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Functional characterization and modified rescue of novel AE1 mutation R730C associated with overhydrated cation leak stomatocytosis.American journal of physiology. Cell physiology · 2011Article
- Article
- Structures of membrane proteins.Quarterly reviews of biophysics · 2010Review
- Visualizing the mapped ion pathway through the Na,K-ATPase pump.Channels (Austin, Tex.) · 2009Review
- Ion channels versus ion pumps: the principal difference, in principle.Nature reviews. Molecular cell biology · 2009Review
- Introduction. The blurred boundary between channels and transporters.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
In principle, an ion channel needs no more than a single gate, but a pump requires at least two gates that open and close alternately to allow ion access from only one side of the membrane at a time. In the Na+,K+-ATPase pump, this alternating gating effects outward transport of three Na+ ions and inward transport of two K+ ions, for each ATP hydrolysed, up to a hundred times per second, generating a measurable current if assayed in millions of pumps. Under these assay conditions, voltage jumps elicit brief charge movements, consistent with displacement of ions along the ion pathway while one gate is open but the other closed. Binding of the marine toxin, palytoxin, to the Na+,K+-ATPase uncouples the two gates, so that although each gate still responds to its physiological ligand they are no longer constrained to open and close alternately, and the Na+,K+-ATPase is transformed into a gated cation channel. Millions of Na+ or K+ ions per second flow through such an open pump-channel, permitting assay of single molecules and allowing unprecedented access to the ion transport pathway through the Na+,K+-ATPase. Use of variously charged small hydrophilic thiol-specific reagents to probe cysteine targets introduced throughout the pump's transmembrane segments allows mapping and characterization of the route traversed by transported ions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.