ArticleMicroscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada2008
Second-harmonic generation imaging of membrane potential with photon counting.
Article in Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Second harmonic imaging of membrane potentials: Comparing water and chromophores as probes.Biophysical journal · 2026Article
- Second harmonic generation microscopy: a powerful tool for bio-imaging.Biophysical reviews · 2023Review
- Phagosomal chloride dynamics in the alveolar macrophage.iScience · 2022Article
- Second Harmonic Generation Spectroscopy of Membrane Probe Dynamics in Gram-Positive Bacteria.Biophysical journal · 2019Article
- Porphyrin Dyes for Nonlinear Optical Imaging of Live Cells.iScience · 2018Article
- Porphyrins for probing electrical potential across lipid bilayer membranes by second harmonic generation.Angewandte Chemie (International ed. in English) · 2013Article
- Single cell optical imaging and spectroscopy.Chemical reviews · 2013Review
- Optical recording of action potentials in mammalian neurons using a microbial rhodopsin.Nature methods · 2011Article
- Biological applications of second harmonic imaging.Biophysical reviews · 2011Review
- Imaging voltage in neurons.Neuron · 2011Review
- Optophysiological approach to resolve neuronal action potentials with high spatial and temporal resolution in cultured neurons.Frontiers in cellular neuroscience · 2011Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Second-harmonic generation (SHG) can be used for imaging membrane potential in neurons, but poor signal-to-noise (S/N) limits accurate measurements of small voltage transients. We use photon counting to improve the S/N of weak SHG signal detection. Photon counting generates shot-noise limited and integrable signals, eliminates pulse-to-pulse variation, and built-in discriminators reduces the background to practically zero. In single trials, by using photon counting, we obtain a more than a twofold S/N increase over analog voltage detection. Trial-to-trial variability is also reduced by 50%. Finally, we show that, using photon counting, the kinetics of fast events such as action potentials can be recorded more accurately.
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Registered trials
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