ArticleBMC musculoskeletal disorders2008
Differential gene expression associated with postnatal equine articular cartilage maturation.
Article in BMC musculoskeletal disorders, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
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- Donor age effects on in vitro chondrogenic and osteogenic differentiation performance of equine bone marrow- and adipose tissue-derived mesenchymal stromal cells.BMC veterinary research · 2022Article
- TRPV4 activation enhances compressive properties and glycosaminoglycan deposition of equine neocartilage sheets.Osteoarthritis and cartilage open · 2022Article
- Rejuvenation of extensively passaged human chondrocytes to engineer functional articular cartilage.Biofabrication · 2021Article
- Kinetics of Gene Expression Changes in Equine Fetal Interzone and Anlagen Cells Over 14 Days of Induced Chondrogenesis.Frontiers in veterinary science · 2021Article
- Adding exogenous biglycan or decorin improves tendon formation for equine peritenon and tendon proper cells in vitro.BMC musculoskeletal disorders · 2020Article
- Tenascin-C expression controls the maturation of articular cartilage in mice.BMC research notes · 2020Article
- Cellular Proliferation of Equine Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells Decline With Increasing Donor Age.Frontiers in veterinary science · 2020Article
- Changes in the appendicular skeleton during metamorphosis in the axolotl salamander (Ambystoma mexicanum).Journal of anatomy · 2018Article
- Articular cartilage and joint development from embryogenesis to adulthood.Seminars in cell & developmental biology · 2017Review
- Articular Cartilage: Structural and Developmental Intricacies and Questions.Current osteoporosis reports · 2015Review
- Transcriptomic signatures in cartilage ageing.Arthritis research & therapy · 2013Article
- Different responses to mechanical injury in neonatal and adult ovine articular cartilage.Biomedical engineering online · 2013Article
- Collagen V is a dominant regulator of collagen fibrillogenesis: dysfunctional regulation of structure and function in a corneal-stroma-specific Col5a1-null mouse model.Journal of cell science · 2011Article
- Transcriptional profiling differences for articular cartilage and repair tissue in equine joint surface lesions.BMC medical genomics · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundArticular cartilage undergoes an important maturation process from neonate to adult that is reflected by alterations in matrix protein organization and increased heterogeneity of chondrocyte morphology. In the horse, these changes are influenced by exercise during the first five months of postnatal life. Transcriptional profiling was used to evaluate changes in articular chondrocyte gene expression during postnatal growth and development.
methodsTotal RNA was isolated from the articular cartilage of neonatal (0-10 days) and adult (4-5 years) horses, subjected to one round of linear RNA amplification, and then applied to a 9,367-element equine-specific cDNA microarray. Comparisons were made with a dye-swap experimental design. Microarray results for selected genes (COL2A1, COMP, P4HA1, TGFB1, TGFBR3, TNC) were validated by quantitative polymerase chain reaction (qPCR).
resultsFifty-six probe sets, which represent 45 gene products, were up-regulated (p < 0.01) in chondrocytes of neonatal articular cartilage relative to chondrocytes of adult articular cartilage. Conversely, 586 probe sets, which represent 499 gene products, were up-regulated (p < 0.01) in chondrocytes of adult articular cartilage relative to chondrocytes of neonatal articular cartilage. Collagens, matrix-modifying enzymes, and provisional matrix non-collagenous proteins were expressed at higher levels in the articular cartilage of newborn foals. Those genes with increased mRNA abundance in adult chondrocytes included leucine-rich small proteoglycans, matrix assembly, and cartilage maintenance proteins.
conclusionDifferential expression of genes encoding matrix proteins and matrix-modifying enzymes between neonates and adults reflect a cellular maturation process in articular chondrocytes. Up-regulated transcripts in neonatal cartilage are consistent with growth and expansion of the articular surface. Expression patterns in mature articular cartilage indicate a transition from growth to homeostasis, and tissue function related to withstanding shear and weight-bearing stresses.
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