ArticleMolecular therapy : the journal of the American Society of Gene Therapy2009
Shielding of sleeping beauty DNA transposon-delivered transgene cassettes by heterologous insulators in early embryonal cells.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Construction and Quantitative Evaluation of a Tissue-Specific Sleeping Beauty by EDL2-Specific Transposase Expression in Esophageal Squamous Carcinoma Cell Line KYSE-30.Molecular biotechnology · 2023Article
- Episomes and Transposases-Utilities to Maintain Transgene Expression from Nonviral Vectors.Genes · 2022Review
- Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element (UCOE).BMC biotechnology · 2019Article
- Preclinical and clinical advances in transposon-based gene therapy.Bioscience reports · 2017Review
- The KRAB zinc finger protein ZFP809 is required to initiate epigenetic silencing of endogenous retroviruses.Genes & development · 2015Article
- Sleeping Beauty transposon system for genetic etiological research and gene therapy of cancers.Cancer biology & therapy · 2015Review
- DNA transposon-based gene vehicles - scenes from an evolutionary drive.Journal of biomedical science · 2013Review
- Gene therapy for hemoglobinopathies: progress and challenges.Translational research : the journal of laboratory and clinical medicine · 2013Review
- Efficient sleeping beauty DNA transposition from DNA minicircles.Molecular therapy. Nucleic acids · 2013Article
- Optimization of the piggyBac transposon using mRNA and insulators: toward a more reliable gene delivery system.PloS one · 2013Article
- The impact of cHS4 insulators on DNA transposon vector mobilization and silencing in retinal pigment epithelium cells.PloS one · 2012Article
- Pig transgenesis by Sleeping Beauty DNA transposition.Transgenic research · 2011Article
- The Sleeping Beauty transposon system: a non-viral vector for gene therapy.Human molecular genetics · 2011Review
- Erythroid-specific expression of β-globin from Sleeping Beauty-transduced human hematopoietic progenitor cells.PloS one · 2011Article
- Hybrid lentiviral vectors.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Review
- Helper-independent piggyBac plasmids for gene delivery approaches: strategies for avoiding potential genotoxic effects.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Article
- PiggyBac transposon-based inducible gene expression in vivo after somatic cell gene transfer.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Genome-wide mapping of PiggyBac transposon integrations in primary human T cells.Journal of immunotherapy (Hagerstown, Md. : 1997) · 2009Article
- Beyond the rat models of human neurodegenerative disorders.Cellular and molecular neurobiology · 2009Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Sleeping Beauty (SB) transposon system represents an important alternative to viral integrating vector systems but may, as its viral counterparts, be subject to transcriptional silencing. To investigate shielding of SB-delivered transgene cassettes against transcriptional repression, we establish silencing assays in which SB vector-containing F9 murine teratocarcinoma cell clones are identified by strategies that include or exclude selection for transgene expression. Among clones carrying one or more SB transposon vectors, more than one-third are immediately silenced, and most of the remaining clones move toward silencing during prolonged passage. In line with the lack of an intrinsic ability of SB to resist silencing, we show that the stable transfection rate of SB vectors in F9 cells is significantly improved by flanking the transgene with heterologous 5'-HS4 chicken beta-globin (cHS4) insulators. In approaches based on drug selection and subsequent flow-cytometric detection of transgene expression, clones containing cHS4-insulated vectors are to a much higher degree protected against transcriptional silencing, resulting in long-term expression of the fluorescent marker. Our findings demonstrate that SB vectors, prone for transcriptional silencing by positional effects in F9 cells, are protected by insulators. We believe that insulated SB-derived vectors will become useful tools in transposon-based transgenesis and therapeutic gene transfer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.