ArticleActa veterinaria Scandinavica2008
In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence.
Article in Acta veterinaria Scandinavica, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Understanding the regulation of protein synthesis under stress conditions.Biophysical journal · 2024Article
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- IRES-mediated Pichia pastoris cell-free protein synthesis.Bioresources and bioprocessing · 2023Article
- Immunoinformatics and molecular dynamics approaches: Next generation vaccine design against West Nile virus.PloS one · 2021Article
- Improved Homologous Expression of the Acidic Lipase fromJournal of microbiology and biotechnology · 2020Article
- Codon-Optimized P1A-Encoding DNA Vaccine: Toward a Therapeutic Vaccination against P815 Mastocytoma.Molecular therapy. Nucleic acids · 2017Article
- DNA-epitope vaccine provided efficient protection to mice against lethal dose of influenza A virus H1N1.Viral immunology · 2014Article
- Synthetic DNA vaccines: improved vaccine potency by electroporation and co-delivered genetic adjuvants.Frontiers in immunology · 2013Review
- DNA-vaccine platform development against H1N1 subtype of swine influenza A viruses.Viral immunology · 2012Article
- Article
- DNA vaccines for targeting bacterial infections.Expert review of vaccines · 2010Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the prerequisite for developing DNA vaccines for horses are vectors that are efficiently expressed in horse cells. We have analysed the ectopic expression of the human serum albumin gene in primary horse cells from different tissues. The vectors used are of pcDNA and pUC origin and include the cytomegalovirus (CMV) promoter. The pUC vectors contain CMV intron A whereas the pcDNA vectors do not. Insertion of intron A diminished the expression from the pcDNA vectors whereas insertion of a Kozak sequence upstream of the gene in two types of pUC vectors increased significantly the in vitro expression in primary horse cells derived from skin, lung, duodenum and kidney. We report for the first time the significance of full consensus Kozak sequences for protein expression in horse cells in vitro.
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