ArticlePlant molecular biology2009
cDNA cloning and functional characterisation of CYP98A14 and NADPH:cytochrome P450 reductase from Coleus blumei involved in rosmarinic acid biosynthesis.
Article in Plant molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 82 citations in OpenAlex.
- Cytochrome P450 enzymes in the phenylpropanoid pathway: CYP73 and CYP98 candidate genes in Chara braunii, Anthoceros agrestis, Marchantia polymorpha and Physcomitrium patens.The Plant journal : for cell and molecular biology · 2026Article
- Temporal Phenolic Profile and Bioactivity of EndemicAntioxidants (Basel, Switzerland) · 2026Article
- Combining Stable Isotope Labeling and Candidate Substrate-Product Pair Networks Reveals Lignan, Oligolignol, and Chicoric Acid Biosynthesis in Flax Seedlings (Plants (Basel, Switzerland) · 2025Article
- Review
- CYP98A monooxygenases: a key enzyme family in plant phenolic compound biosynthesis.Horticulture research · 2025Article
- The chromosome-scale assembly of the Salvia plebeia genome provides insight into the biosynthesis and regulation of rosmarinic acid.Plant biotechnology journal · 2025Article
- Reinforced NADPH regeneration in engineered Saccharomyces cerevisiae enhances rosmarinic acid production.Archives of microbiology · 2025Article
- Phenolic metabolism in Sarcandra glabra is mediated by distinct BAHD hydroxycinnamoyltransferases.The Plant journal : for cell and molecular biology · 2025Article
- Tuning architectural organization of eukaryotic P450 system to boost bioproduction in Escherichia coli.Nature communications · 2024Article
- Versatile CYP98A enzymes catalyse meta-hydroxylation reveals diversity of salvianolic acids biosynthesis.Plant biotechnology journal · 2024Article
- Mining and functional characterization of NADPH-cytochrome P450 reductases of the DNJ biosynthetic pathway in mulberry leaves.BMC plant biology · 2024Article
- Systematic characterization of gene families and functional analysis ofFrontiers in plant science · 2024Article
- Cloning and Functional Characterization of NADPH-Cytochrome P450 Reductases inMolecules (Basel, Switzerland) · 2023Article
- Complete biosynthesis of the phenylethanoid glycoside verbascoside.Plant communications · 2023Article
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- Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid.Antioxidants (Basel, Switzerland) · 2020Review
- Metabolic Engineering ofACS synthetic biology · 2020Article
- Functional characterization of NADPH-cytochrome P450 reductase and cinnamic acid 4-hydroxylase encoding genes from Scoparia dulcis L.Botanical studies · 2020Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The final reactions of rosmarinic acid biosynthesis, the introduction of the aromatic 3- and 3'-hydroxyl groups, are catalysed by cytochrome P450-dependent hydroxylases. The cDNAs encoding CYP98A14 as well as a NADPH:cytochrome P450 reductase (CPR) were isolated from Coleus blumei and actively expressed in Saccharomyces cerevisiae. The CYP98A14-cDNA showed an open reading frame of 1521 nucleotides with high similarities to 4-coumaroylshikimate/quinate 3-hydroxylases. Yeast microsomes harbouring the CYP98A14 protein catalysed the 3-hydroxylation of 4-coumaroyl-3',4'-dihydroxyphenyllactate and the 3'-hydroxylation of caffeoyl-4'-hydroxyphenyllactate, in both cases forming rosmarinic acid. Apparent K (m)-values for 4-coumaroyl-3',4'-dihydroxyphenyllactate and caffeoyl-4'-hydroxyphenyllactate were determined to be at 5 microM and 40 microM, respectively. CYP98A14 differs from CYP98s from other plants, since 4-coumaroylshikimate or -quinate were not accepted as substrates. Coexpression of the Coleus blumei CPR and CYP98A14 in the same yeast cells increased the hydroxylation activity up to sevenfold. CYP98A14 from Coleus blumei is a novel bifunctional cytochrome P450 specialised for rosmarinic acid biosynthesis.
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