ArticleChromosoma2009
Subnuclear compartmentalization and function of actin and nuclear myosin I in plants.
Article in Chromosoma, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- Nuclear actin is a critical regulator ofbioRxiv : the preprint server for biology · 2024Article
- Bioinformatics Analysis of Actin Interactome: Characterization of the Nuclear and Cytoplasmic Actin-Binding Proteins.The protein journal · 2024Article
- Prostaglandins limit nuclear actin to control nucleolar function during oogenesis.Frontiers in cell and developmental biology · 2023Article
- Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography.Light, science & applications · 2021Article
- New Insights into Cellular Functions of Nuclear Actin.Biology · 2021Review
- Indirect visualization of endogenous nuclear actin by correlative light and electron microscopy (CLEM) using an actin-directed chromobody.Histochemistry and cell biology · 2019Article
- Nuclear Actin: From Discovery to Function.Anatomical record (Hoboken, N.J. : 2007) · 2018Review
- Multiple Pools of Nuclear Actin.Anatomical record (Hoboken, N.J. : 2007) · 2018Article
- NMCP/LINC proteins: putative lamin analogs in plants?Plant signaling & behavior · 2013Review
- ARP2/3 localization in Arabidopsis leaf pavement cells: a diversity of intracellular pools and cytoskeletal interactions.Frontiers in plant science · 2013Article
- The nucleoskeleton as a genome-associated dynamic 'network of networks'.Nature reviews. Molecular cell biology · 2011Review
- Differential sublocalization of actin variants within the nucleus.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus.Histochemistry and cell biology · 2010Article
- A single vegetative actin isovariant overexpressed under the control of multiple regulatory sequences is sufficient for normal Arabidopsis development.The Plant cell · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actins are highly conserved proteins that serve as the basic building blocks of cytoskeletal microfilaments. In animal cells, specific nuclear actin adopts unconventional conformations that are involved in multiple nuclear functions and that associate with nuclear actin binding proteins. However, there is practically no information available about nuclear actin in plants. Indeed, actin has not been detected in the nuclear proteomes of many plants, and orthologs of the main structural nuclear actin-binding proteins have yet to be identified. Here, we have investigated the characteristics, intranuclear compartmentalization, and function of actin in isolated Allium cepa nuclei as well as that of its motor protein nuclear myosin I (NMI). Using conformation-specific antibodies for nuclear actin isoforms, ss-actin, and NMI, the distribution of these proteins was studied in Western blots and by immunocytochemistry. Moreover, the participation of nuclear actin in transcription was analyzed in run on in situ assays and inhibition of RNA polymerases I and II. We show that actin isoforms with distinct solubilities are present in onion nuclei with a consistent subnuclear compartmentalization. Actin and NMI are highly enriched in foci that are similar to transcription foci, although actin is also distributed diffusely in the nucleus and nucleolus as well as accumulating in a subset of the Cajal bodies. Immunogold labeling identified both proteins in the nuclear transcription subdomains and in other subnuclear compartments. In addition, actin and NMI were diffusely distributed in the nuclear matrix.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.