ArticleMethods in molecular biology (Clifton, N.J.)2008
RNA affinity tags for the rapid purification and investigation of RNAs and RNA-protein complexes.
Article in Methods in molecular biology (Clifton, N.J.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed.
- Chemical Biology 2025: Highlights From the Ch/Bi145 Course at Caltech.Chembiochem : a European journal of chemical biology · 2026Review
- Engineering covalent small molecule-RNA complexes in living cells.Nature chemical biology · 2025Article
- A Novel Method to Isolate RNase MRP Using RNA Streptavidin Aptamer Tags.Bio-protocol · 2023Article
- Characterization of epitranscriptome reader proteins experimentally andComputational and structural biotechnology journal · 2023Review
- NMR characterization of RNA small molecule interactions.Methods (San Diego, Calif.) · 2019Review
- Directed Evolution of Split APEX2 Peroxidase.ACS chemical biology · 2019Article
- Exploring ribosome composition and newly synthesized proteins through proteomics and potential biomedical applications.Expert review of proteomics · 2017Review
- Purification of Transcript-Specific mRNP Complexes Formed In Vivo from Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Generation of Recombinant Polioviruses Harboring RNA Affinity Tags in the 5' and 3' Noncoding Regions of Genomic RNAs.Viruses · 2016Article
- Purification of Messenger Ribonucleoprotein Particles via a Tagged Nascent Polypeptide.PloS one · 2016Article
- RNA sequences required for the noncoding function of oskar RNA also mediate regulation of Oskar protein expression by Bicoid Stability Factor.Developmental biology · 2015Article
- oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction.RNA (New York, N.Y.) · 2015Article
- A Platform for Discovery and Quantification of Modified Ribonucleosides in RNA: Application to Stress-Induced Reprogramming of tRNA Modifications.Methods in enzymology · 2015Article
- Electrophilic activity-based RNA probes reveal a self-alkylating RNA for RNA labeling.Nature chemical biology · 2014Article
- Advances in methods for native expression and purification of RNA for structural studies.Current opinion in structural biology · 2014Review
- Multi-disciplinary methods to define RNA-protein interactions and regulatory networks.Current opinion in genetics & development · 2013Review
- MS2-TRAP (MS2-tagged RNA affinity purification): tagging RNA to identify associated miRNAs.Methods (San Diego, Calif.) · 2012Article
- Translational regulation of the cell cycle: when, where, how and why?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- mTOR phosphorylates IMP2 to promote IGF2 mRNA translation by internal ribosomal entry.Genes & development · 2011Article
- Efficient detection of RNA-protein interactions using tethered RNAs.Nucleic acids research · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Isolation of ribonucleoprotein particles from living cells and cell lysates has allowed the identification of both simple bimolecular interactions and the members of large, extended complexes. A number of different strategies have been devised to isolate these complexes by using affinity purification methods that are specific for the RNA rather than the protein components of these complexes. We describe the use of two such RNA affinity tags: small RNAs that bind with high affinity and specificity to either Sephadex beads or streptavidin affinity resins and can be eluted under mild, native conditions that retain intact complexes. The tags can be inserted into appropriate locations in genes encoding the RNA components, and ribonucleoproteins can be assembled either in vivo or in vitro before affinity isolation. Strategies toward the design and production of these tagged RNA sequences are discussed, and the purification procedure is outlined.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.